The effect of casein phosphopeptide-amorphous calcium phosphate-propolis chewing gum on calcium and phosphate ion levels in caries-free subject’s saliva and streptococcus mutans biofilm formation

Sri Angky Soekanto, Gadia Canaparimita Ghrena Duhita, Endang Winiati, Muhamad Sahlan

Research output: Contribution to journalArticlepeer-review

5 Citations (Scopus)

Abstract

Objective: The aim of this study is to analyze the amount of calcium and phosphate ion released by casein phosphopeptide-amorphous calcium phosphate (CPP-ACP), which supports the remineralization and the effectiveness of propolis wax in suppressing the mass formation of Streptococcus mutans biofilm on caries-free subjects and to observe if these two active components are effective when combined into one substance and placed inside sugar-free chewing gum. Methods: Twenty-five samples of caries-free saliva before and after the mastication simulation (five concentrations of chewing gum) in vitro were observed to check for release of calcium and phosphate ions along with a biofilm assay. The release of calcium ions was detected using AAS, the release of phosphate ions was detected using spectrophotometry ultraviolet-visible, and the biofilm assay was detected using a crystal violet 96-well plate ELISA and was evaluated with an ELISA reader. Result: Chewing gum with a concentration of 0% prop + 5% CPP-ACP showed the highest release level of calcium ions (p<0.05) and phosphate ions (p>0.05) and is significant in suppressing the mass formation of the S. mutans biofilm (p<0.05). Conclusions: Mastication simulation of CPP-ACP-Propolis chewing gum can increase calcium and phosphate ion levels in caries-free saliva and decrease S. mutans biofilm mass formation; this can support remineralization and becomes a dental caries prevention alternative.

Original languageEnglish
Pages (from-to)174-176
Number of pages3
JournalAsian Journal of Pharmaceutical and Clinical Research
Volume10
Issue numberSpecial Issue October
DOIs
Publication statusPublished - 1 Oct 2017

Keywords

  • Biofilm
  • Caries
  • Casein phosphopeptide-amorphous calcium phosphate
  • Propolis wax
  • Saliva

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