The effect of addition glutathione antioxidant on vitrification medium to the viability of Garut sheep (Ovis aries) oocytes

M. Gunawan, P. A. Pratiwi, E. M. Kaiin, L. Sjahfirdi

Research output: Contribution to journalConference articlepeer-review

Abstract

Research has been conducted to investigate the viability of Garut sheep oocyte to evaluate the effect of glutathione antioxidants addition with a concentration of 0.5 mM, 1 mM, and 1.5 mM in the vitrification medium to the viability of Garut sheep oocytes. The oocytes was freezed at -196 °C for 7 days. A total of 135 oocytes first were matured in vitro with the addition of 1 mM glutathione for 24 hours. The oocyte were vitrified using 15 % ethylene glycol, 15 % dimethyl sulfoxide, 0.5 M sucrose and divided into four treatments group with the addition of 0 mM (KK), 0.5 mM (KP 1), 1 mM (KP 2), and 1.5 mM (KP 3) glutathione. Viability was evaluated through the luminescence produced by Hoechst and Propidium Iodide dyes. The percentage of viable oocytes are 69.70 % (0 mM), 72.73 % (0.5 mM), 76.47 % (1 mM), and 77.14 % (1.5 mM). The results tends to increase along with the addition of glutathione concentration. However, statistically, ANOVA were not significantly different among treatment groups (P > 0.05). The conclusion of the study is that the addition of glutathione antioxidants in the vitrification medium are able to maintain the viability of Garut sheep oocytes during vitrification, although the ANOVA test were not significant.

Original languageEnglish
Article number012049
JournalJournal of Physics: Conference Series
Volume1725
Issue number1
DOIs
Publication statusPublished - 12 Jan 2021
Event2nd Basic and Applied Sciences Interdisciplinary Conference 2018, BASIC 2018 - Depok, Indonesia
Duration: 3 Aug 20184 Aug 2018

Keywords

  • Garut sheep
  • Glutathione
  • Oocyte
  • Viability
  • Vitrification

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