The effect of addition alpha tocopherol antioxidant on slow freezing medium to the quality of Garut sheep oocyte (Ovis aries)

E. M. Kaiin, E. N. Salmah, M. Gunawan, L. Sjahfirdi

Research output: Contribution to journalConference articlepeer-review

Abstract

This study has evaluated the ability of alpha tocopherol antioxidant in maintaining the quality of Garut sheep oocytes post-cryopreservation using slow freezing method. Total of 226 oocytes with two different qualities (A and B) are matured in vitro in TCM-199 medium added with 150 μM alpha tocopherol. Afterwards, the oocytes were cryopreserved in the slow freezing medium with the addition of 0 μM (KK), 100 μM (KP1), 150 μM (KP2) and 200 μM (KP3) alpha tocopherol. Combination of cryoprotectant 10 % ethylene glycol (EG) and 0.1 M sucrose were also added to the medium slow freezing. Oocyte evaluation was carried out after 7 days of storage in liquid nitrogen (-196 °C), including oocyte morphology and oocyte viability using Hoechst and Propidium Iodide (PI) dyes. Based on the results of the study, the percentage of normal oocyte post-cryopreservation are 0 μM (67.86 %), 100 μM (68.42 %), 150 μM (74.58 %) and 200 μM (61.11 %). The percentage of viable oocyte post-cryopreservation are 0 μM (76.79 %), 100 μM (80.70 %), 150 μM (86.44 %), and 200 μM (70.37 %). The results shows that the addition of alpha tocopherol did not have an effect to the quality of oocytes post-cryopreservation, but there was a tendency for quality improvement with increasing doses of alpha tocopherol. In this study, addition of 150 μM alpha tocopherol into slow freezing medium is the best dose for maintaining the quality of Garut sheep oocytes until post-cryopreservation, although not significant (P > 0.05).

Original languageEnglish
Article number012064
JournalJournal of Physics: Conference Series
Volume1725
Issue number1
DOIs
Publication statusPublished - 12 Jan 2021
Event2nd Basic and Applied Sciences Interdisciplinary Conference 2018, BASIC 2018 - Depok, Indonesia
Duration: 3 Aug 20184 Aug 2018

Keywords

  • Alpha tocopherol
  • Garut sheep
  • Oocyte viability
  • Slow freezing

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