The effect of 70% Ethanolic extract of Dayak Onion Bulbs and Cowpea on Cardiovascular Parameters of Hypoestrogen Model Rats

Introduction: Eleutherinol has strong affinity to bind with Estrogen Receptor alpha (ER-α) and can be found in Dayak onion bulbs (Eleutherine bulbosa (Mill.) Urb.). The cowpea (Vigna unguiculata (L.) Walp) contain Daidzein that acts on estrogen receptors beta (ER-β). This study was aimed to investigate the effect of both extracts on ER-α and ER-β by estimating the blood pressure and serum lipid profile of hypoestrogen model rats. Material and Methods: Thirty-six of female Sprague-Dawley were randomly assigned to eight groups as followed, SHAM, OVX, RAL (Raloxifene 1 mg/200 g BW), Cowpea 100 mg/200 g BW with Dayak onion bulbs: 36 mg/200 g BW (D1); 18 mg/200 g BW (D2); 9 mg/200 g BW (D3); 4.5 mg/200 g BW (D4) and single dose of cowpea 100 mg/200 g BW (D5). All groups, except the SHAM,were ovariectomized to obtain the conditions of hypoestrogen. The extracts were given orally for 28 days. Blood pressure and lipid profile were measured after 28 days of treatment. Results: Combination of extracts at D2 (18 mg/200 g BW Dayak onion bulbs and 100 mg/200 g BW cowpea) significantly decreased blood pressure and serum lipid profile of hypoestrogen model rats. The combination extracts was also better than a single dose of 100 mg/200 g BW cowpea. Conclusions: The results indicated that a combination extract of Dayak onion bulbs and cowpea could be used as a drug for menopause woman in the future.