The Cilutung River has an essential role in meeting water needs, especially in Sumedang and Majalengka Districts. The drought that has occurred in the Cilutung watershed in recent years indicates a need to monitor the availability of water in the watershed. This study aimed to determine the annual water availability in the Cilutung watershed in 2009 and 2019 related to the dynamics of land cover change. The land cover maps in 2009 and 2019 were obtained from the Landsat series imagery, classified into 6 (six) land cover classes. The method used to calculate water availability and water demand is the InVEST Water Yield Model, a hydrological method with a water balance approach. The calculation results show that rainfall and vegetation cover positively correlate with water availability, while the increase in built-up land has a positive correlation with water demand. In 2009 Sub DAS 7 had high water yields compared to other sub-watersheds, but in 2019 the highest water yield was produced by Sub DAS 6. Due to the significant increase in built-up land in Sub-watershed 7, water demand also increased. Sub-watersheds with high water yield can select priority areas for water resource conservation to maintain the water supply in the Cilutung watershed.