The Differences of Fusion (F) Gene Nucleotide Sequence and F-Protein Amino Acid Sequence of Wild Measles Virus and Vaccine Virus in Indonesia

Measles virus is one of the cause of disease in babies and children. There are 2 glycoproteins in the virus envelope, hemaglutinin protein and fusion protein. The F protein of measles virus is essential for the virus to infect cells. This research was aimed to know the amino acid sequence differences of nucleotide and amino acid sequence of F protein between the wild-type measles (G2, G3, and D9), the CAM 70 vaccine, the Schwarz, and the Edmonston-wt viruses. The extraction and amplification of the gene were conducted in the laboratory using biomoleculer technology. The gene analysis was conducted using bioinformatic technology. We found that the biggest diffferences of nucleotide sequence between the wild-type measles and the CAM-70 vaccine virus was 83-90 nucleotides; the Schwarz virus was 16-23 nucleotides, and the Edmonston-wt was 18-23 nucleotides. The biggest amino acid sequence difference (29-31 residus) was found between the wild measles and CAM-70 vaccine virus, and the Schwarz virus (4-5 residus) and the Edmonston-wt virus (1-3 residus). It was concluded that there were differences of nucleotide sequence and F protein amnio acid between the wild measles virus and the vaccine virus in Indonesia. The wild type measles virus had a closer genetic relation to the Schwarz vaccine virus compared to the CAM-70 vaccine virus.