TY - JOUR
T1 - The Differences of Amino Acid Sequence in Hemaglutinin Protein Between The Wild-Type Measles Virus and The Vaccine Virus in Indonesia
AU - Setiawan, Made
AU - Sjahrurachman, Agus
AU - Ibrahim, R. Fera
AU - Suwandono, Agus
PY - 2009
Y1 - 2009
N2 - The H-protein of the measles virus is an important part of the virus to infect the host. The glycosilation and receptor sites of H-protein are considered to be essential in the process of infecting the cells and immune receptor. If there is a defect in important-site amino acid of the Hprotein, the infection process in the host cells and the immune respons will be disturbed. The aimed of this research is to know the differences of important-site amino acid sequence of H-protein between the wild-type measles virus (G2, G3, and D9), the CAM-70 vaccine virus, the Schwarz vaccine virus, and the Edmonston-wt virus. The extraction and amplification of the gene were conducted in the laboratory using biomolecular technology. The gene and protein analysis were conducted using the bioinformatic technology. We found glycosilation site differences between the H-protein of the G3 genotype wild measles virus with other wild measles virus and the vaccine virus. The biggest CD46-contact site differences are found between the wild measles virus (G2, G3, and D9) and CAM-70, compared to Schwarz and Edmonston-wt. We do not find the amino acid sequence differences in the CD 150 contact site. We conclude that the Indonesian wild measles virus has an amino acid sequence difference at the glycosilation and CD 46 contact site, between the wild measles virus and the CAM-70.
AB - The H-protein of the measles virus is an important part of the virus to infect the host. The glycosilation and receptor sites of H-protein are considered to be essential in the process of infecting the cells and immune receptor. If there is a defect in important-site amino acid of the Hprotein, the infection process in the host cells and the immune respons will be disturbed. The aimed of this research is to know the differences of important-site amino acid sequence of H-protein between the wild-type measles virus (G2, G3, and D9), the CAM-70 vaccine virus, the Schwarz vaccine virus, and the Edmonston-wt virus. The extraction and amplification of the gene were conducted in the laboratory using biomolecular technology. The gene and protein analysis were conducted using the bioinformatic technology. We found glycosilation site differences between the H-protein of the G3 genotype wild measles virus with other wild measles virus and the vaccine virus. The biggest CD46-contact site differences are found between the wild measles virus (G2, G3, and D9) and CAM-70, compared to Schwarz and Edmonston-wt. We do not find the amino acid sequence differences in the CD 150 contact site. We conclude that the Indonesian wild measles virus has an amino acid sequence difference at the glycosilation and CD 46 contact site, between the wild measles virus and the CAM-70.
UR - http://indonesia.digitaljournals.org/index.php/idnmed/article/view/622
M3 - Article
SN - 2654-3796
JO - Journal of the Indonesian Medical Association : Majalah Kedokteran Indonesia
JF - Journal of the Indonesian Medical Association : Majalah Kedokteran Indonesia
ER -