The detection of veillonella spp. in the oral mucosa of indonesian high-risk early childhood caries by absolute quantitative real-time PCR

The presence of oral Veillonela spp., V. atypica, V. denticariosi, V. dispar, V. parvula and V. rogosae, in oral mucosa biofilms and saliva have been associated with dental cary development in young children. However, identifying oral Veillonella spp. is difficult since there is no useful differential phenotypic test. To reliably detect Veillonella-related early childhood caries (ECC), we used a real-time PCR assay based on SYBR Green I, followed by a melting curve analysis. The q-PCR identified the oral Veillonella spp. directly from the clinical specimen. The oral mucosa biofilm collected from ECC was dominated by V. dispar, while the minor species was V. rogosae. This is the first report indicating the profile of oral Veillonella spp. in the oral mucosa biofilm of high-risk EEC in Indonesian subjects.