TY - GEN
T1 - The Cryoprotecting Effects of Trehalose and Dimethyl Sulfoxide on Alginate-Chitosan Encapsulated Mesenchymal Stem Cells
AU - Nurhayati, Retno Wahyu
AU - Pratama, Gita
AU - Dinastri, Muftia
N1 - Funding Information:
This work was supported by P5 (Program Pendanaan Perancangan dan Pengembangan Purwarupa) No. PKS-13/UN2.INV/HKP.05/2020 Research Grant from Universitas Indonesia awarded to Dr. Retno Wahyu Nurhayati.
Publisher Copyright:
© 2022 American Institute of Physics Inc.. All rights reserved.
PY - 2022/8/16
Y1 - 2022/8/16
N2 - Trehalose is a promising cryoprotectant for the long-term storage of mesenchymal stem cells (MSCs). Trehalose is relatively safe for the human body and a potential substitute to reduce the toxicity of dimethyl sulfoxide (DMSO), a common cryoprotectant. Previously, we developed an alginate-chitosan microcapsule containing human stem cells. However, the strategy for long-term storage of microencapsulated cells is not established yet. The current study was conducted to evaluate the potencies of trehalose and DMSO for cryopreservation of MSC-loaded alginate-chitosan microcapsules. The microcapsules were preserved with trehalose and/or DMSO and then stored in an isopropanol container at -80°C for stimulating a controlled cooling process. After a week of cryopreservation, microscopic observations showed that cryopreserved microcapsules maintained similar morphologies with freshly-prepared microcapsules. The only physical difference was a slight shrinking of the outer chitosan layer in the cryopreserved microcapsule, which was probably due to dehydration. Among cryopreserved microcapsules, DMSO 10% treatment gave the highest viability (88%), and then a combination of trehalose (50 mM) + DMSO (2%) treatment was 75%, and trehalose only treatment was 50%. DMSO (2%) treatment was excluded due to uncommon low cell recovery. Altogether, it was concluded that trehalose (50 mM) could be used to lower DMSO concentration, whereas low DMSO concentration (2%) or trehalose (50mM) alone was not sufficient to protect microencapsulated MSCs from negative effects of freezing and thawing. This study provides brief evidence of trehalose and DMSO potencies for cryopreserving stem cell-loaded microcapsule.
AB - Trehalose is a promising cryoprotectant for the long-term storage of mesenchymal stem cells (MSCs). Trehalose is relatively safe for the human body and a potential substitute to reduce the toxicity of dimethyl sulfoxide (DMSO), a common cryoprotectant. Previously, we developed an alginate-chitosan microcapsule containing human stem cells. However, the strategy for long-term storage of microencapsulated cells is not established yet. The current study was conducted to evaluate the potencies of trehalose and DMSO for cryopreservation of MSC-loaded alginate-chitosan microcapsules. The microcapsules were preserved with trehalose and/or DMSO and then stored in an isopropanol container at -80°C for stimulating a controlled cooling process. After a week of cryopreservation, microscopic observations showed that cryopreserved microcapsules maintained similar morphologies with freshly-prepared microcapsules. The only physical difference was a slight shrinking of the outer chitosan layer in the cryopreserved microcapsule, which was probably due to dehydration. Among cryopreserved microcapsules, DMSO 10% treatment gave the highest viability (88%), and then a combination of trehalose (50 mM) + DMSO (2%) treatment was 75%, and trehalose only treatment was 50%. DMSO (2%) treatment was excluded due to uncommon low cell recovery. Altogether, it was concluded that trehalose (50 mM) could be used to lower DMSO concentration, whereas low DMSO concentration (2%) or trehalose (50mM) alone was not sufficient to protect microencapsulated MSCs from negative effects of freezing and thawing. This study provides brief evidence of trehalose and DMSO potencies for cryopreserving stem cell-loaded microcapsule.
KW - alginate
KW - chitosan
KW - cryopreservation
KW - dimethyl sulfoxide
KW - mesenchymal stem cells
KW - microcapsule
KW - trehalose
KW - umbilical cord
UR - http://www.scopus.com/inward/record.url?scp=85138292980&partnerID=8YFLogxK
U2 - 10.1063/5.0107055
DO - 10.1063/5.0107055
M3 - Conference contribution
AN - SCOPUS:85138292980
T3 - AIP Conference Proceedings
BT - 6th Biomedical Engineering''s Recent Progress in Biomaterials, Drugs Development, and Medical Devices
A2 - Rahman, Siti Fauziyah
A2 - Zakiyuddin, Ahmad
A2 - Whulanza, Yudan
A2 - Intan, Nurul
PB - American Institute of Physics Inc.
T2 - 6th International Symposium of Biomedical Engineering''s Recent Progress in Biomaterials, Drugs Development, and Medical Devices, ISBE 2021
Y2 - 7 July 2021 through 8 July 2021
ER -