TY - JOUR
T1 - The correlation between urinary 8-iso-prostaglandin F2α and hydrogen peroxide toward renal function in T2DM patients consuming sulfonylurea and combination of metformin-sulfonylurea
AU - Sauriasari, Rani
AU - Wulandari, Fitri
AU - Nurifahmi, Rahmaningtyas
AU - Sekar, Andisyah P.
AU - Susilo, Veronika Y.
N1 - Funding Information:
This study was supported by PUPT grant Ministry of Research, Technology and Higher Education, Indonesia for the financial support and DRPM (Directorate of Research and Community Engagement) Universitas Indonesia for their assistance.
Publisher Copyright:
© 2018 Bentham Science Publishers.
PY - 2018/4/1
Y1 - 2018/4/1
N2 - Background: Renal dysfunction is a common complication in type 2 diabetes mellitus patients associated with oxidative damage which could be characterized by 8-iso-prostaglandin F2α and hydrogen peroxide level as oxidative stress markers. Objective: The aim of our study is to determine if there is a difference in 8-iso-prostaglandin F2α and hydrogen peroxide levels between sulfonylurea and combination of metformin-sulfonylurea in diabetic patients. We also wanted to determine if these oxidative stress markers correlate with the estimated Glomerular Filtration Rate (eGFR). Subjects and Methods: We conducted a cross-sectional study with inclusion of 55 patients with type 2 diabetes mellitus in Dr. Sitanala Tangerang Hospital, Indonesia with purposive sampling. The value of eGFR was obtained by serum creatinine levels, while the level of 8-iso-prostaglandin F2α was measured by ELISA and urinary hydrogen peroxide using FOX-1 (Ferrous Ion Oxidation Xylenol Orange 1). Results: There was no difference in 8-iso-prostaglandin F2α and hydrogen peroxide level between the two groups (p=0.088 and p=0.848). Moreover, there was no difference in eGFR values between the two groups, measured by Cockroft-Gault, MDRD, and CKD-EPI. 8-iso-prostaglandin F2α(n=55) was positively correlated with eGFR based on Cockroft-Gault (r=0.382; p=0.009), whereas urinary hydrogen peroxide (n=47) also generate significant positive correlation with eGFR based on the MDRD equation (r=0.326; p=0.021). Linear regression analysis showed that 8-iso-prostaglandin F2α is the most predictive factor and the only significant factor for eGFR in Cockroft-Gault, MDRD and also CKD-EPI, even after controlled by gender, age, BMI, HbA1c, systole, and H2O2. Conclusion: The two treatments did not have any significant differences in antioxidant activity. However, an increase of urinary 8-iso-prostaglandin F2α and hydrogen peroxide which correlates with eGFR in the total sample may play a significant role in the pathophysiology of diabetic nephropathy.
AB - Background: Renal dysfunction is a common complication in type 2 diabetes mellitus patients associated with oxidative damage which could be characterized by 8-iso-prostaglandin F2α and hydrogen peroxide level as oxidative stress markers. Objective: The aim of our study is to determine if there is a difference in 8-iso-prostaglandin F2α and hydrogen peroxide levels between sulfonylurea and combination of metformin-sulfonylurea in diabetic patients. We also wanted to determine if these oxidative stress markers correlate with the estimated Glomerular Filtration Rate (eGFR). Subjects and Methods: We conducted a cross-sectional study with inclusion of 55 patients with type 2 diabetes mellitus in Dr. Sitanala Tangerang Hospital, Indonesia with purposive sampling. The value of eGFR was obtained by serum creatinine levels, while the level of 8-iso-prostaglandin F2α was measured by ELISA and urinary hydrogen peroxide using FOX-1 (Ferrous Ion Oxidation Xylenol Orange 1). Results: There was no difference in 8-iso-prostaglandin F2α and hydrogen peroxide level between the two groups (p=0.088 and p=0.848). Moreover, there was no difference in eGFR values between the two groups, measured by Cockroft-Gault, MDRD, and CKD-EPI. 8-iso-prostaglandin F2α(n=55) was positively correlated with eGFR based on Cockroft-Gault (r=0.382; p=0.009), whereas urinary hydrogen peroxide (n=47) also generate significant positive correlation with eGFR based on the MDRD equation (r=0.326; p=0.021). Linear regression analysis showed that 8-iso-prostaglandin F2α is the most predictive factor and the only significant factor for eGFR in Cockroft-Gault, MDRD and also CKD-EPI, even after controlled by gender, age, BMI, HbA1c, systole, and H2O2. Conclusion: The two treatments did not have any significant differences in antioxidant activity. However, an increase of urinary 8-iso-prostaglandin F2α and hydrogen peroxide which correlates with eGFR in the total sample may play a significant role in the pathophysiology of diabetic nephropathy.
KW - 8-iso-prostaglandin F
KW - Estimated glomerular filtration rate
KW - Metformin
KW - Sulfonylurea
KW - Type 2 diabetes mellitus
UR - http://www.scopus.com/inward/record.url?scp=85045987903&partnerID=8YFLogxK
U2 - 10.2174/1573399812666161214141716
DO - 10.2174/1573399812666161214141716
M3 - Article
C2 - 27981897
AN - SCOPUS:85045987903
VL - 14
SP - 189
EP - 195
JO - Current Diabetes Reviews
JF - Current Diabetes Reviews
SN - 1573-3998
IS - 2
ER -