The Combination of Spirulina Extract and Sambiloto Effect Histopathologically on Medial Colon from Plasmodium berghei Anka Infected Mice

Introduction: Malaria is one of the biggest burden in medical section in Indonesia, as the prevalence is still high and half of the provinces in Indonesia considered as endemic area.^1-4 Moreover, the drug resistant case number has grown larger within years.^3-7 Indonesian people are very close with traditional drug regiment that derived from plants, for example Sambiloto and Spirulina.^7-9 This research done in order to see the effect of Sambiloto and Spirulina combination from histopathologic aspect in medial colon of P. berghei infected mice. Method: The data taken from experimental study using male Swiss Webster mice that has been infected with Plasmodium berghei Anka. The four groups of mice were given different treatment. The first group treated with Sambiloto only, the second one with Sambiloto and Spirulina extract, the third group with Sambiloto and Spirulina powder and the last one the control group with administration of DHP. Results: The result shows that the group with extract and powder spirulina show a significant result in the inflammatory focus and angiogenesis. However, this research does not necessarily prove the correlation between Sambiloto-Spirulina and their effect on the goblet cell and dysplasia grade on the infected mice, as the result for both category is insignificant. Conclusion: The study showed that spirulina has positive effect on inflamatory focus and angiogenesis, but the goblet cells count and dysplasia grade result is not noteworthy, as it requires prolonged inflammation process in order to achieve the optimal result.