Preservation of animals and human ovarian tissue by vitrification has been developed to find the efficient methods to overcome the damaging effect from cold shock. The aim of this study is to evaluate the effect of combination of ethylene glycol (EG) as intracellular cryoprotectant and palm dates juice as extracellular cryoprotectant at concentration 3.75 %, 7.5 % and 15 %, respectively with ratio 1:1 in preserving the ovarian tissue after 48 hours vitrification, based on ovarian weight. The ovaries of 21 mature Sprague-Dawley rats in the proestrus stage were isolated and weighed. Ovaries of normal control group (n = 3) were not vitrified, control treatment group (n = 9) was submerged in ethylene glycol (EG) solution and treatment group (n = 9) was submerged in combination of ethylene glycol and palm dates juice solution with three different concentrations inside the cryotube and were continued with vitrification or stored in liquid nitrogen (-196 °C) for 48 hours, then thawed and re-weighed. The result showed that there was no significant difference between experimental groups on pre-vitrification and post-vitrification ovarian weight (P > 0.05). In conclusion, combination of ethylene glycol and palm dates juice with three different concentrations does not cause significant effect on the ovarian tissue post-vitrification.