Rice is a commodity which production continues to be increased in Indonesia due to the increasing demand and consumption per capita. The use of intensive agriculture and inorganic fertilizers that leave residues on the land makes fertilizers ineffective. Tobacco plants commonly used as pesticides can be used as organic materials that can increase rice growth because they contain nutrients and various allelochemical compounds. This study aims to determine the effect of tobacco extract application at various concentrations and interval combination treatments on growth parameters consisting of plant height rate, number of tillers, number of leaves, root dry weight, shoot dry weight, shoot-root ratio, flowering period, and the concentration of phytohormones (IAA and GA3) in rice (Oryza sativa) variety Inpari 32. The study was conducted by the application of tobacco extract consisting of 9 treatments which were a combination of concentration (1-3ml/L) and intervals application (1x in 1-3 weeks) and one control (without the administration of tobacco extract) for a total of 10 treatments, with three replications of each treatment. The results showed that the combination of concentration and interval of applications of tobacco extract had no significant effect on the parameters of plant height, tiller number, leaf number, shoot dry weight, flowering period, and phytohormones (IAA and GA3) concentrations in rice (Oryza sativa) Inpari 32 variety, and affect the parameters of dry root weight and shoot-root ratio slightly.