The combination effect 6 % of glycerol and skim milk on spermatozoa motility of giant grouper Epinephelus lanceolatus (Bloch 1970) after frozen

A. K. Sudrajat, A. Abinawanto, R. Lestari, A. Bowolaksono, N. G. Zavitri

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

The objective of present study was to investigate the combination effect of 6 % of glycerol and skim milk on spermatozoa motility of giant grouper, Epinephelus lanceolatus (Bloch 1970) cryopreserved for 48 hours. The ejaculates were diluted with the solvent (fish ringer extender+6 % glycerol+skim milk), the dilution ratio between sperm and diluent solution is 1:9. Samples were then equilibrated for 10 minutes at 4 °C and were frozen for 48 hours at -20 °C. Thawing was carried out for 30 seconds at 45 °C. Data were analyzed using by Anova and Tukey test. According to result ANOVA test, combination of 6 % glycerol with skim milk in spermatozoa motility after frozen had a significant effect (P > 0.05). Further, based on Tukey test, 15 %, 20 %, and 25 % of skim milk was demonstrated significantly different with control and the highest spermatozoa motility was 20 % (80.51±3.46 %).

Original languageEnglish
Title of host publicationProceedings of the 4th International Symposium on Current Progress in Mathematics and Sciences, ISCPMS 2018
EditorsTerry Mart, Djoko Triyono, Ivandini T. Anggraningrum
PublisherAmerican Institute of Physics Inc.
ISBN (Electronic)9780735419155
DOIs
Publication statusPublished - 4 Nov 2019
Event4th International Symposium on Current Progress in Mathematics and Sciences 2018, ISCPMS 2018 - Depok, Indonesia
Duration: 30 Oct 201831 Oct 2018

Publication series

NameAIP Conference Proceedings
Volume2168
ISSN (Print)0094-243X
ISSN (Electronic)1551-7616

Conference

Conference4th International Symposium on Current Progress in Mathematics and Sciences 2018, ISCPMS 2018
CountryIndonesia
CityDepok
Period30/10/1831/10/18

Keywords

  • Cryopreservation
  • Epinephelus lanceolatus
  • skim milk
  • spermatozoa motility

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    Sudrajat, A. K., Abinawanto, A., Lestari, R., Bowolaksono, A., & Zavitri, N. G. (2019). The combination effect 6 % of glycerol and skim milk on spermatozoa motility of giant grouper Epinephelus lanceolatus (Bloch 1970) after frozen. In T. Mart, D. Triyono, & I. T. Anggraningrum (Eds.), Proceedings of the 4th International Symposium on Current Progress in Mathematics and Sciences, ISCPMS 2018 [020094] (AIP Conference Proceedings; Vol. 2168). American Institute of Physics Inc.. https://doi.org/10.1063/1.5132521