@inproceedings{a0a1edeac9184a94bdcf0dc2917f4041,
title = "The combination effect 6 % of glycerol and skim milk on spermatozoa motility of giant grouper Epinephelus lanceolatus (Bloch 1970) after frozen",
abstract = "The objective of present study was to investigate the combination effect of 6 % of glycerol and skim milk on spermatozoa motility of giant grouper, Epinephelus lanceolatus (Bloch 1970) cryopreserved for 48 hours. The ejaculates were diluted with the solvent (fish ringer extender+6 % glycerol+skim milk), the dilution ratio between sperm and diluent solution is 1:9. Samples were then equilibrated for 10 minutes at 4 °C and were frozen for 48 hours at -20 °C. Thawing was carried out for 30 seconds at 45 °C. Data were analyzed using by Anova and Tukey test. According to result ANOVA test, combination of 6 % glycerol with skim milk in spermatozoa motility after frozen had a significant effect (P > 0.05). Further, based on Tukey test, 15 %, 20 %, and 25 % of skim milk was demonstrated significantly different with control and the highest spermatozoa motility was 20 % (80.51±3.46 %).",
keywords = "Cryopreservation, Epinephelus lanceolatus, skim milk, spermatozoa motility",
author = "Sudrajat, {A. K.} and A. Abinawanto and R. Lestari and A. Bowolaksono and Zavitri, {N. G.}",
note = "Publisher Copyright: {\textcopyright} 2019 Author(s).; 4th International Symposium on Current Progress in Mathematics and Sciences 2018, ISCPMS 2018 ; Conference date: 30-10-2018 Through 31-10-2018",
year = "2019",
month = nov,
day = "4",
doi = "10.1063/1.5132521",
language = "English",
series = "AIP Conference Proceedings",
publisher = "American Institute of Physics Inc.",
editor = "Terry Mart and Djoko Triyono and Anggraningrum, {Ivandini T.}",
booktitle = "Proceedings of the 4th International Symposium on Current Progress in Mathematics and Sciences, ISCPMS 2018",
address = "United States",
}