The Changes of HIF-1a and ICAM-1 Expression after Miana (Coleus Scutellariodes [L]) Treatment in Balb/C Mice with Mycobacterium Tuberculosis Infection

Rosamarlina, Mochammad Hatta, Irawaty Djaharuddin, Ilhamjaya Patellongi, Agus Dwi Susanto, Andi Asadul Islam, Muhammad Nasrum Massi, Agussalim Bukhari, Arif Santoso, Nur Ahmad Tabri, Farida Murtiani, Ade Rifka Junita, Ahmad Syukri Saleh, Ressy Dwiyanti, Sesilia Rante Pakadang

Research output: Contribution to journalArticlepeer-review

1 Citation (Scopus)

Abstract

Increasing resistance to TB drugs raises the challenge of TB eradication. Miana leaves is Indonesian traditional herbal medicine, have antimicrobial, anti-inflammatory, and immunoregulatory action. Not much is known about the effect of Miana on HIF-1a and ICAM-1, the immunoregulators of infection and inflammation. This study aims to elucidate the effect of Miana on HIF-1a and ICAM-1 in M. tuberculosis (Mtb) infected mice. This experimental study used Mtb infected Balb/c mice were divided into 4 groups; group 1 is placebo, group 2 is treated with Rifampicin as Anti TB drug, group 3 is treated with Miana, and group 4 is treated with Miana + Anti TB drug. HIF-1a and ICAM-1 serum levels were analyzed using ELISA. There is a significant difference of mean HIF-1a (p= 0.00, F = 114.21) and ICAM-1 (p= 0.00, F = 113.11) between the four groups after treatment. HIF-1a level is significantly lower in anti TB treatment, Miana, and Miana + anti TB treatment compared to placebo (mean difference (MD) 35,764.67, p=0.00; 29,230.98, p=0.000; 38,489.62, p=0.00, respectively). Furthermore, ICAM-1 level is significantly lower in anti TB treatment, Miana, and Miana + anti TB treatment compared to placebo (MD 95,449.68, p=0.00; 79,509.69, p=0.00; 108,672.83, p=0.00, respectively). HIF-1a and ICAM-1 expression was reduced after Miana administration. Miana can be a potential complement to anti-TB treatment but cannot replace rifampicin as anti-TB drugs.

Original languageEnglish
Pages (from-to)73-81
Number of pages9
JournalBiomedical and Pharmacology Journal
Volume15
Issue number1
DOIs
Publication statusPublished - 2022

Keywords

  • Coleus scutellariodes
  • HIF-1a
  • ICAM-1
  • M. tuberculosis
  • Miana leaf
  • Rifampicin

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