The apoptotic effect of gallic acid and its derivatives on primary cultured endometriosis cells

Arleni Bustami, Hajar Dachniar, Zoya Marie Adyasa, Salma Suka Kyana Nareswari, Popi Sopiah, Ade Arsianti, Raden Muharram Natadisastra

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2 Citations (Scopus)


Background: Research into endometriosis falls behind despite increasing publication for the last 5 years, contributing to lack of non-invasive treatments and 20% decrease in quality of life. Since gallic acid usage as anti-inflammatory agent has been elucidated in cancer cells, this study serves to investigate the potential of gallic acid as an apoptotic inducer in endometriosis cells. Methods: Primary culture of endometriosis was derived from patients who had laparascopy via enzymatic technique. In vitro endometriosis cells were treated with three dosages of 25.6 g/ml, 51.2 g/ml and 102.4 g/ml of gallic acid, heptyl gallate, and octyl gallate for 48 hours. Determination of quality and quantification of early, late, viable, and necrotic cells was done using confocal fluorescence with acridine orange/ethidium bromide staining of at least 100 cells per sample. Results: Control samples showed 63.8% cell underwent apoptosis. Gallic acid, heptyl gallate, and octyl gallate showed different inhibition pattern. Apoptosis after gallic acid treatment decreased from 90.1% to 79.2% as the dose is increased. On the contrary, 51.2 g/ml heptyl induce 92.5% apoptosis, while octyl showed most apoptosis at 93.1% Conclusion: This study exhibited apoptotic inductor effect of octyl gallate, followed by heptyl gallate and gallic acid and their potency as treatment for endometriosis.

Original languageEnglish
Pages (from-to)6697-6700
Number of pages4
JournalAdvanced Science Letters
Issue number7
Publication statusPublished - Jul 2017


  • AO/EB staining
  • Apoptosis
  • Confocal fluorescence
  • Endometrial cells
  • Endometriosis
  • Gallic acid
  • Heptyl gallate
  • Octyl gallate


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