The antioxidant effects of curcumin on rat liver mitochondrial dysfunction induced by tert-butylhydroperoxide

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

This study was designed to investigate the protective effects of curcumin on rat liver mitochondria dysfunction induced by tert-butylhydroperoxide (t-BuOOH). The mitochondrial preparation was homogeneously purified as shown by the relative specific activity of succinate dehydrogenase (SDH) and cytochrome coxidase (CCO) of 72 and 33, respectively. Incubation of the mitochondrial preparation with 80 μmol mg protein-1 t-BuOOH resulted in a substantial increase of thiobarbituric acid reactive substances (279%), 20% decrease of SDH and CCO activities, 68% decrease of GSH and 667% increase of GSSG, in comparison to control; whereas superoxide dismutase activity was not affected. Curcumin of 5 μM could prevent the loss of SDH and CCO activities caused by t-BuOOH. Loss of GSH due to t-BuOOH, however, could only be partially prevented by 500 mM of curcumin. This study shows a potent antioxidant activity of curcumin in preventing mitochondrial dysfunction caused by t-BuOOH.

Original languageEnglish
Pages (from-to)145-151
Number of pages7
JournalJournal of Ecophysiology and Occupational Health
Volume4
Issue number3-4
Publication statusPublished - 1 Jan 2004

Keywords

  • Curcumin
  • Mitochondria
  • Tert-butylhydroperoxide

Fingerprint Dive into the research topics of 'The antioxidant effects of curcumin on rat liver mitochondrial dysfunction induced by tert-butylhydroperoxide'. Together they form a unique fingerprint.

  • Cite this