This study was designed to investigate the protective effects of curcumin on rat liver mitochondria dysfunction induced by tert-butylhydroperoxide (t-BuOOH). The mitochondrial preparation was homogeneously purified as shown by the relative specific activity of succinate dehydrogenase (SDH) and cytochrome coxidase (CCO) of 72 and 33, respectively. Incubation of the mitochondrial preparation with 80 μmol mg protein-1 t-BuOOH resulted in a substantial increase of thiobarbituric acid reactive substances (279%), 20% decrease of SDH and CCO activities, 68% decrease of GSH and 667% increase of GSSG, in comparison to control; whereas superoxide dismutase activity was not affected. Curcumin of 5 μM could prevent the loss of SDH and CCO activities caused by t-BuOOH. Loss of GSH due to t-BuOOH, however, could only be partially prevented by 500 mM of curcumin. This study shows a potent antioxidant activity of curcumin in preventing mitochondrial dysfunction caused by t-BuOOH.
|Number of pages||7|
|Journal||Journal of Ecophysiology and Occupational Health|
|Publication status||Published - 1 Jan 2004|