Technique for Determining the Viability of Acanthamoeba Cysts Treated with a Cysticidal Agent Based on Membrane Integrity

Nor Farah Azwani Che Mohamad, Nur Syakinah Nafisa Failei, Nurhidayana Mohd Rased, Azila Adnan, Ma Nyuk Ling, Hazlina Ahamad Zakeri, Eny Kusrini, Fatimah Hashim

Research output: Contribution to journalArticlepeer-review


This study presents a straightforward and reliable method for determining the viability of Acanthamoeba cysts. A standard method for determining Acanthamoeba cyst viability in an in vitro cytotoxicity analysis is required to ensure that the double-walled and sturdy cysts are affected by the substance tested. In this study, a new approach was used to determine the cysticidal potential of redox Cleland’s reagent, dithiothreitol (DTT), against Acanthamoeba cysts. This approach constitutes a significant breakthrough, as the cyst form of Acanthamoeba is known for its high resistance to various chemicals and drugs used to treat infections of the central nervous system and eyes caused by Acanthamoeba. Cyst viability was evaluated based on the intensity of the cyst population under fluorescence produced by propidium iodide (PI) dye and measured using an enzyme-linked immunosorbent assay (ELISA) reader at an absorbance of 636 nm. The results were validated using high-content screening (HCS). For analysis, an individual cell was imaged and examined for phenotypic changes in the Acanthamoeba cyst at the cyst population level. Fluorescence intensity of the cysts in each well in a 96-well plate was measured using Image J software. HCS is an automated technique that uses fluorescence microscopy to produce quantitative data.

Original languageEnglish
Pages (from-to)720-727
Number of pages8
JournalInternational Journal of Technology
Issue number4
Publication statusPublished - Oct 2021


  • Dithiothreitol
  • Fluorescence intensity
  • High content screening
  • Keratitis
  • Propidium iodide


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