T cell receptor (TCR) gene rearrangement during the course of chronic hepatitis B. A preliminary report

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Abstract

T cells recognize specific antigens through the T cell receptor (TCR) and self MHC molecules. Structural variation in the TCR to recognise the enormous diversity of antigens is effected by, maturation rearrangement of TCR genes in a manner analogous to immunoglobulin genes. Although it is believed that T cells play a major role in the seroconversion of e Ag positive hepatitis B carriers, the mechanisms are still unclear. To examine the role of TCR rearrangements in chronic hepatitis B we have carried out Southern transfers of DNA aliquots from peripheral blood mononuclear cells (PBMC) usitrg TCR chain specific probes. Rearrangements affecting as few as 5% of PBMC could. be detected. Rearrangements were more common in e Ag positive than negative. Of several rearrangement patterns a 5 kb EcoRl and 5.5 kb Hind III were most common. Longitudinal studies of 6 patients suggested the appearence of the rearrangement band preceded seroconversion and could be detected in PBMC for 6 months after seroconversion. It is postulated that during seroconversion the appearance of a clone of T cells with a particular rearrangement is associated with seroconversion and characterisation of these clones is currently being undertaken.

Original languageEnglish
Pages (from-to)127-135
Number of pages9
JournalMedical Journal of Indonesia
Volume3
Issue number3
DOIs
Publication statusPublished - 1 Jul 1994

Keywords

  • Chronic hepatitis B
  • Gene rearrrangement
  • T cell receptor

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