Objective: In this study, we aimed to create complexes consisting of copper and manganese mineral elements and amino acids. Methods: We determined the mineral element levels both bound and in their free state using atomic absorption spectrophotometry. The synthesized complexes were characterized using infrared spectrophotometry, while the free and bound mineral contents were separated using ion exchange chromatography. Results: Our results demonstrated that the synthesis of such complexes was successful. The free mineral contents of the copper-methionine, coppertryptophan, and manganese-methionine complexes were 4.52, 6.53, and 0.056 mg/kg, respectively, while the bound mineral contents of the coppermethionine, copper-tryptophan, manganese-methionine, and manganese-tryptophan complexes were 96.885, 114.974, 57.778, and 49.624 mg/kg, respectively. Conclusion: The synthesis of copper and manganese complexes were successfully formed and analysis.
- Atomic absorption spectrophotometry
- Ion exchange chromatography column