Synthesis, characterization, and cytotoxicity evaluation of gallic acid nanoparticles towards breast T47D cancer cells

Ade Arsianti, Anton Bahtiar, Fadilah Fadilah, Vincent Kharisma Wangsaputra, Rafika Indah Paramita, Norma Nur Azizah, Lince Dameria Nadapdap, Ajeng Megawati Fajrin, Hiroki Tanimoto, Kiyomi Kakiuchi

Research output: Contribution to journalArticle

Abstract

Introduction: Gallic acid is a naturally polyphenolic acid which shows cytotoxicity against several cancer cells, as well as it displays chemo-preventive activity which is attributed to its strong apoptosis- inducing and antioxidant effects. Thus, gallic acid has become an attractive substance to be further developed due to its strong cytotoxic activity. This study aimed to synthesize gallic acid nanoparticle coating with alginate-chitosan, and evaluate its cytotoxicity against breast T47D cancer cells. Methods: Gallic acid nanoparticle was synthesized using ionic gelation method. The yield, size and morphology of the nanoparticles were determined by UV-Vis Spectroscopy, Transmission electron microscopy (TEM) and Fourier Transform Infrared (FTIR) spectroscopy. Cytotoxicity evaluation of gallic acid nanoparticle towards breast T47D cancer cell is carried out by MTT(3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazoliumbromide) assay. Results: Spherical nanoparticles of gallic acid with the size of 100-200 nm has been successfully synthesized in 96% of yield. Compared to gallic acid (IC50: 20.86 µg/mL) and alginate-chitosan nanoparticle (IC50: 38.46 µg/mL), gallic acid coating with alginate-chitosan nanoparticles demonstrated higher cytotoxicity towards breast T47D cancer cells with IC50 value of 9.03µg/mL. Conclusion: Our results clearly confirmed that gallic acid nanoparticles coating with alginate-chitosan showed a strong cytotoxicity towards breast T47D cancer cells, which is potential to be developed as a candidate for new anti-breast cancer agent.

Original languageEnglish
Pages (from-to)321-327
Number of pages7
JournalPharmacognosy Journal
Volume12
Issue number2
DOIs
Publication statusPublished - 1 Jan 2020

Keywords

  • Cytotoxicity
  • Gallic acid
  • Nanoparticle
  • Synthesis
  • T47D cells

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    Arsianti, A., Bahtiar, A., Fadilah, F., Wangsaputra, V. K., Paramita, R. I., Azizah, N. N., Nadapdap, L. D., Fajrin, A. M., Tanimoto, H., & Kakiuchi, K. (2020). Synthesis, characterization, and cytotoxicity evaluation of gallic acid nanoparticles towards breast T47D cancer cells. Pharmacognosy Journal, 12(2), 321-327. https://doi.org/10.5530/pj.2020.12.51