TY - JOUR
T1 - Synergistic effect of trichloroethylene and Cu(II) on DNA-adduct 8-hydroxy deoxyguanosine (8-OHdG) formation as a biomarker of cancer risk
AU - Nurhidayat, I.
AU - Budiawan, null
N1 - Publisher Copyright:
© Published under licence by IOP Publishing Ltd.
PY - 2021/6/14
Y1 - 2021/6/14
N2 - Identification of the synergistic effect of trichloro ethylene and Cu (II) in the formation of DNA adduct 8-hydroxy deoxyguanosine (8-OHdG) as a cancer biomarker were carried out. TCE and CuCl2 were incubated with deoxyguanosine with variations in temperature (37°C and 60°C) and pH (7.4 and 8.4) for 6 hours. The 8-OHdG levels formed were analyzed using high performance liquid chromatography (HPLC). The formation of 8-OHdG from exposure to TCE seems to require high concentrations, because of its difficulty to oxidize. The addition of Cu (II) was shown to have a synergistic effect with TCE in the formation of DNA adducts which increased up to 65.8%. Temperature did not have a significant effect on DNA adduct formation. Furthermore, it is found that alkaline pH produces more DNA adducts than neutral pH.
AB - Identification of the synergistic effect of trichloro ethylene and Cu (II) in the formation of DNA adduct 8-hydroxy deoxyguanosine (8-OHdG) as a cancer biomarker were carried out. TCE and CuCl2 were incubated with deoxyguanosine with variations in temperature (37°C and 60°C) and pH (7.4 and 8.4) for 6 hours. The 8-OHdG levels formed were analyzed using high performance liquid chromatography (HPLC). The formation of 8-OHdG from exposure to TCE seems to require high concentrations, because of its difficulty to oxidize. The addition of Cu (II) was shown to have a synergistic effect with TCE in the formation of DNA adducts which increased up to 65.8%. Temperature did not have a significant effect on DNA adduct formation. Furthermore, it is found that alkaline pH produces more DNA adducts than neutral pH.
UR - http://www.scopus.com/inward/record.url?scp=85108552108&partnerID=8YFLogxK
U2 - 10.1088/1742-6596/1918/3/032011
DO - 10.1088/1742-6596/1918/3/032011
M3 - Conference article
AN - SCOPUS:85108552108
SN - 1742-6588
VL - 1918
JO - Journal of Physics: Conference Series
JF - Journal of Physics: Conference Series
IS - 3
M1 - 032011
T2 - 7th International Conference on Mathematics, Science, and Education 2020, ICMSE 2020
Y2 - 6 October 2020 through 6 October 2020
ER -
