TY - JOUR
T1 - Surface plasmon resonance analysis for detecting non-structural protein 1 of dengue virus in Indonesia
AU - Widoretno,
AU - Sjahrurachman, Agus
AU - Dewi, Beti Ernawati
AU - Lischer, Kenny
AU - Pratami, Diah Kartika
AU - Flamandita, Darin
AU - Sahlan, Muhamad
N1 - Funding Information:
The authors would like to thank the Indonesia Research Partnership on Infectious Disease (INA-RESPOND) for the helpful discussion to finish this research. We would like to also acknowledge DRPM Universitas Indonesia Grant Publikasi Terindeks Internasional (PUTI) Q1 2020 (Grant No. NKB-1406/UN2.RST/HKP.05.00/2020).
Publisher Copyright:
© 2020 The Authors
PY - 2020/8
Y1 - 2020/8
N2 - Dengue is an acute febrile disease caused by dengue virus (DENV) that is transmitted by Aedes sp., which causes serious health conditions in many countries. Non-structural protein 1 (NS1) is a co-factor for the RNA replication of this virus, which represents a new strategy for the identification of dengue. Prompt and accurate laboratory diagnosis of this infection is required to assist in patient triage and management, as well as prevent the spread of this infection. In the present study, we tested the potential of surface plasmon resonance (SPR) as a diagnostic tool for dengue infections. NS1 antigen protein was used as an analyte that targets anti-NS1 antibodies, with their interaction resulting in a change in the refractive index. In comparison to currently available gold-standard detection methods [enzyme-linked immunosorbent assay (ELISA) and reverse transcription polymerase chain reaction (RT-PCR)], SPR showed a similar sensitivity but greater efficiency and simplicity in terms of infection detection. Out of 26 samples collected from patients with dengue in Indonesia, SPR was able to correctly identify all 16 positively infected individuals at a lower concentration and a shorter period of time compared to ELISA and RT-PCR. This study revealed that SPR is a promising tool for DENV detection and potentially other diseases as well.
AB - Dengue is an acute febrile disease caused by dengue virus (DENV) that is transmitted by Aedes sp., which causes serious health conditions in many countries. Non-structural protein 1 (NS1) is a co-factor for the RNA replication of this virus, which represents a new strategy for the identification of dengue. Prompt and accurate laboratory diagnosis of this infection is required to assist in patient triage and management, as well as prevent the spread of this infection. In the present study, we tested the potential of surface plasmon resonance (SPR) as a diagnostic tool for dengue infections. NS1 antigen protein was used as an analyte that targets anti-NS1 antibodies, with their interaction resulting in a change in the refractive index. In comparison to currently available gold-standard detection methods [enzyme-linked immunosorbent assay (ELISA) and reverse transcription polymerase chain reaction (RT-PCR)], SPR showed a similar sensitivity but greater efficiency and simplicity in terms of infection detection. Out of 26 samples collected from patients with dengue in Indonesia, SPR was able to correctly identify all 16 positively infected individuals at a lower concentration and a shorter period of time compared to ELISA and RT-PCR. This study revealed that SPR is a promising tool for DENV detection and potentially other diseases as well.
KW - Alternative method
KW - Anti-non-structural protein 1
KW - Dengue detection
KW - Non-structural protein 1
KW - Surface plasmon resonance
UR - http://www.scopus.com/inward/record.url?scp=85087012075&partnerID=8YFLogxK
U2 - 10.1016/j.sjbs.2020.06.018
DO - 10.1016/j.sjbs.2020.06.018
M3 - Article
AN - SCOPUS:85087012075
SN - 1319-562X
VL - 27
SP - 1931
EP - 1937
JO - Saudi Journal of Biological Sciences
JF - Saudi Journal of Biological Sciences
IS - 8
ER -
