Suppression of in vivo tumorigenicity of rat hepatoma cell line KDH-8 cells by soluble TGF-β receptor type II

Wenli Zhao, Masonobu Kobayashi, Wei Ding, Lan Yuan, Prem Seth, Santoso Cornain, Jingxin Wang, Futoshi Okada, Masuo Hosokawa

Research output: Contribution to journalArticlepeer-review

17 Citations (Scopus)

Abstract

We have previously reported that transforming growth factor beta (TGF-β) produced by rat hepatoma cell line KDH-8 cells suppressed the interleukin-2 (IL-2) production of T cells and the tumoricidal activity of macrophages in KDH-8 tumor-bearing rats and that the inhibition of TGF-β production by low-dose bleomycin restored these activities significantly. In this study, we established three transfectant clones with stable expression of soluble TGF-β receptor type II (sTRII), namely KT1, KT2 and KT3, and one with an empty vector used as control vector (KV), and then investigated the effects of sTRII on the tumorigenicity of KDH-8 cells and immune responses in syngeneic Wistar King Aptekman/Hok (WKAH) rats. We found that sTRII expressed in sTRII transfectants could abolish growth inhibition of Mv1Lu cells by TGF-β1 produced by the cells themselves, and that tumor growth of KT2 and KT3 clones in vivo was suppressed significantly compared with that of parent, KV and KT1 clones. Furthermore, we demonstrated that IL-2 production of splenocytes and IL12p40 mRNA expression in tumor tissues were restored in rats inoculated with KT2 and KT3 clones, whereas such restoration was not observed in rats inoculated with parent, KV and KT1 clones. Combined with a low expression of sTRII in KT1 tumor tissues, these results suggest that sTRII may to some extent be able to abolish the tumor-promoting activity of TGF-β, and imply that sTRII might have a therapeutic effect on TGF-β-producing tumors.

Original languageEnglish
Pages (from-to)381-388
Number of pages8
JournalCancer Immunology, Immunotherapy
Volume51
Issue number7
DOIs
Publication statusPublished - 20 Sep 2002

Keywords

  • Immunorestoration
  • Rat hepatocarcinoma
  • TGF-β
  • TGF-β receptor type II

Fingerprint Dive into the research topics of 'Suppression of in vivo tumorigenicity of rat hepatoma cell line KDH-8 cells by soluble TGF-β receptor type II'. Together they form a unique fingerprint.

Cite this