Superoxide detection by chemiluminescence, fluorescence, and spectrophotometry methods

Anindya P. Susanto; Mohammad Firdhaosh; Dong An Wang; Kyaw Thu Moe

doi:10.1109/ICBPE.2006.348652

Superoxide detection by chemiluminescence, fluorescence, and spectrophotometry methods

Anindya P. Susanto, Mohammad Firdhaosh, Dong An Wang, Kyaw Thu Moe

Faculty of Medicine

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › peer-review

2 Citations (Scopus)

Abstract

Pathology of vessel wall endothelium has been attributed to the presence of reactive oxygen species (ROS) and its effects during the onset of oxidative stress. The root of all ROS produced is superoxide (O₂^.-). Hence, its detection is imperative in filtering out appropriate antioxidants and obtaining deeper knowledge on its role. The present study used lucigenin-enhanced chemiluminescence, hydroethidine fluorescence, and cytochrome c spectrophotometric assays to measure O₂^.-. The aim was to compare and contrast among the methods using human embryonic kidney cells (HEK). Superoxide production was induced by NADH via NAD(P)H oxidase system. The study showed that lucigenin enhanced chemiluminescence assay was the most sensitive method whilst hydroethidine and cytochrome c assays were proficient as well.

Original language	English
Title of host publication	ICBPE 2006 - Proceedings of the 2006 International Conference on Biomedical and Pharmaceutical Engineering
Pages	537-540
Number of pages	4
DOIs	https://doi.org/10.1109/ICBPE.2006.348652
Publication status	Published - 2006
Event	ICBPE 2006 - 2006 International Conference on Biomedical and Pharmaceutical Engineering - Singapore, Singapore Duration: 11 Dec 2006 → 14 Dec 2006

Publication series

Name	ICBPE 2006 - Proceedings of the 2006 International Conference on Biomedical and Pharmaceutical Engineering

Conference

Conference	ICBPE 2006 - 2006 International Conference on Biomedical and Pharmaceutical Engineering
Country/Territory	Singapore
City	Singapore
Period	11/12/06 → 14/12/06

Keywords

Cytochrome c
Free radicals
Hydroethidine
Lucigenin
Superoxide

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10.1109/ICBPE.2006.348652

Cite this

Susanto, A. P., Firdhaosh, M., Wang, D. A., & Moe, K. T. (2006). Superoxide detection by chemiluminescence, fluorescence, and spectrophotometry methods. In ICBPE 2006 - Proceedings of the 2006 International Conference on Biomedical and Pharmaceutical Engineering (pp. 537-540). [4155961] (ICBPE 2006 - Proceedings of the 2006 International Conference on Biomedical and Pharmaceutical Engineering). https://doi.org/10.1109/ICBPE.2006.348652

Susanto, Anindya P. ; Firdhaosh, Mohammad ; Wang, Dong An et al. / Superoxide detection by chemiluminescence, fluorescence, and spectrophotometry methods. ICBPE 2006 - Proceedings of the 2006 International Conference on Biomedical and Pharmaceutical Engineering. 2006. pp. 537-540 (ICBPE 2006 - Proceedings of the 2006 International Conference on Biomedical and Pharmaceutical Engineering).

@inproceedings{330460c524854c9082fde8e6ab33792f,

title = "Superoxide detection by chemiluminescence, fluorescence, and spectrophotometry methods",

abstract = "Pathology of vessel wall endothelium has been attributed to the presence of reactive oxygen species (ROS) and its effects during the onset of oxidative stress. The root of all ROS produced is superoxide (O2.-). Hence, its detection is imperative in filtering out appropriate antioxidants and obtaining deeper knowledge on its role. The present study used lucigenin-enhanced chemiluminescence, hydroethidine fluorescence, and cytochrome c spectrophotometric assays to measure O2.-. The aim was to compare and contrast among the methods using human embryonic kidney cells (HEK). Superoxide production was induced by NADH via NAD(P)H oxidase system. The study showed that lucigenin enhanced chemiluminescence assay was the most sensitive method whilst hydroethidine and cytochrome c assays were proficient as well.",

keywords = "Cytochrome c, Free radicals, Hydroethidine, Lucigenin, Superoxide",

author = "Susanto, {Anindya P.} and Mohammad Firdhaosh and Wang, {Dong An} and Moe, {Kyaw Thu}",

year = "2006",

doi = "10.1109/ICBPE.2006.348652",

language = "English",

isbn = "8190426249",

series = "ICBPE 2006 - Proceedings of the 2006 International Conference on Biomedical and Pharmaceutical Engineering",

pages = "537--540",

booktitle = "ICBPE 2006 - Proceedings of the 2006 International Conference on Biomedical and Pharmaceutical Engineering",

note = "ICBPE 2006 - 2006 International Conference on Biomedical and Pharmaceutical Engineering ; Conference date: 11-12-2006 Through 14-12-2006",

}

Susanto, AP, Firdhaosh, M, Wang, DA & Moe, KT 2006, Superoxide detection by chemiluminescence, fluorescence, and spectrophotometry methods. in ICBPE 2006 - Proceedings of the 2006 International Conference on Biomedical and Pharmaceutical Engineering., 4155961, ICBPE 2006 - Proceedings of the 2006 International Conference on Biomedical and Pharmaceutical Engineering, pp. 537-540, ICBPE 2006 - 2006 International Conference on Biomedical and Pharmaceutical Engineering, Singapore, Singapore, 11/12/06. https://doi.org/10.1109/ICBPE.2006.348652

Superoxide detection by chemiluminescence, fluorescence, and spectrophotometry methods. / Susanto, Anindya P.; Firdhaosh, Mohammad; Wang, Dong An et al.

ICBPE 2006 - Proceedings of the 2006 International Conference on Biomedical and Pharmaceutical Engineering. 2006. p. 537-540 4155961 (ICBPE 2006 - Proceedings of the 2006 International Conference on Biomedical and Pharmaceutical Engineering).

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › peer-review

TY - GEN

T1 - Superoxide detection by chemiluminescence, fluorescence, and spectrophotometry methods

AU - Susanto, Anindya P.

AU - Firdhaosh, Mohammad

AU - Wang, Dong An

AU - Moe, Kyaw Thu

PY - 2006

Y1 - 2006

N2 - Pathology of vessel wall endothelium has been attributed to the presence of reactive oxygen species (ROS) and its effects during the onset of oxidative stress. The root of all ROS produced is superoxide (O2.-). Hence, its detection is imperative in filtering out appropriate antioxidants and obtaining deeper knowledge on its role. The present study used lucigenin-enhanced chemiluminescence, hydroethidine fluorescence, and cytochrome c spectrophotometric assays to measure O2.-. The aim was to compare and contrast among the methods using human embryonic kidney cells (HEK). Superoxide production was induced by NADH via NAD(P)H oxidase system. The study showed that lucigenin enhanced chemiluminescence assay was the most sensitive method whilst hydroethidine and cytochrome c assays were proficient as well.

AB - Pathology of vessel wall endothelium has been attributed to the presence of reactive oxygen species (ROS) and its effects during the onset of oxidative stress. The root of all ROS produced is superoxide (O2.-). Hence, its detection is imperative in filtering out appropriate antioxidants and obtaining deeper knowledge on its role. The present study used lucigenin-enhanced chemiluminescence, hydroethidine fluorescence, and cytochrome c spectrophotometric assays to measure O2.-. The aim was to compare and contrast among the methods using human embryonic kidney cells (HEK). Superoxide production was induced by NADH via NAD(P)H oxidase system. The study showed that lucigenin enhanced chemiluminescence assay was the most sensitive method whilst hydroethidine and cytochrome c assays were proficient as well.

KW - Cytochrome c

KW - Free radicals

KW - Hydroethidine

KW - Lucigenin

KW - Superoxide

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U2 - 10.1109/ICBPE.2006.348652

DO - 10.1109/ICBPE.2006.348652

M3 - Conference contribution

AN - SCOPUS:46249132660

SN - 8190426249

SN - 9788190426244

T3 - ICBPE 2006 - Proceedings of the 2006 International Conference on Biomedical and Pharmaceutical Engineering

SP - 537

EP - 540

BT - ICBPE 2006 - Proceedings of the 2006 International Conference on Biomedical and Pharmaceutical Engineering

T2 - ICBPE 2006 - 2006 International Conference on Biomedical and Pharmaceutical Engineering

Y2 - 11 December 2006 through 14 December 2006

ER -

Susanto AP, Firdhaosh M, Wang DA, Moe KT. Superoxide detection by chemiluminescence, fluorescence, and spectrophotometry methods. In ICBPE 2006 - Proceedings of the 2006 International Conference on Biomedical and Pharmaceutical Engineering. 2006. p. 537-540. 4155961. (ICBPE 2006 - Proceedings of the 2006 International Conference on Biomedical and Pharmaceutical Engineering). doi: 10.1109/ICBPE.2006.348652

Superoxide detection by chemiluminescence, fluorescence, and spectrophotometry methods

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Keywords

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