TY - GEN
T1 - Subacute oral toxicity test of chitosan-alginate coated microparticle of garcinia mangostana linn extract
AU - Satriadinatha, Gede Bagus Yoga
AU - Farida, Siti
AU - Mulia, Kamarza
AU - Krisnamurti, Desak Gede Budi
N1 - Funding Information:
This study was supported by National Institute of Health Research and Development, Ministry of Health Republic of Indonesia and Faculty of Medicine as well as Faculty of Engineering, Universitas Indonesia. The authors would also like to thank PITTA (Publikasi Internasional Terindeks untuk Tugas Akhir Mahasiswa) Grant for the financial supports in conducting this study (NKB-0532/UN2.R3.1/HKP.05.00/2019). This grant is presented by Direktorat Riset dan Pengabdian Masyarakat, Universitas Indonesia.
Publisher Copyright:
© 2020 Author(s).
Copyright:
Copyright 2020 Elsevier B.V., All rights reserved.
PY - 2020/5/4
Y1 - 2020/5/4
N2 - Chitosan-alginate coated microparticle of Garcinia mangostana Linn extract promises an alternative treatment of colon cancer patients, as the component of Garcinia mangostana Linn extract has anticancer activity in colon cancer cell lines, and chitosan-alginate could deliver compound faster to colon and accelerate its absorption. However, there have not been any studies evaluating sub-acutely the toxicity effects of this compound on the liver (SGOT and SGPT) and clinical signs of toxicity. This study was conducted to evaluate the subacute toxicity effect of Chitosan-alginate coated microparticle of Garcinia mangostana Linn extract's oral administration. Fifty BALB/c mice were divided into five groups, including doses of 0.5 gram/kg, 1.0 gram/kg, 2.0 gram/kg, negative control, and normal group. Each rat was given an extract by force-feeding via intragastric tube for 14 days (subacute). The results showed that differences in SGOT level (p = 0.061), SGPT level (p = 0.82), and weight gain (p = 0.076) were not significant between the treatment and control groups, accompanied by the absence of clinical signs during the study. These indicate that subacute administration of chitosan-alginate coated microparticle of Garcinia mangostana Linn extract is safe until a dose of 2.0 gram/kgBW. Further studies are needed to evaluate the toxicity effects of this extract on other parameters and in a longer duration of exposure.
AB - Chitosan-alginate coated microparticle of Garcinia mangostana Linn extract promises an alternative treatment of colon cancer patients, as the component of Garcinia mangostana Linn extract has anticancer activity in colon cancer cell lines, and chitosan-alginate could deliver compound faster to colon and accelerate its absorption. However, there have not been any studies evaluating sub-acutely the toxicity effects of this compound on the liver (SGOT and SGPT) and clinical signs of toxicity. This study was conducted to evaluate the subacute toxicity effect of Chitosan-alginate coated microparticle of Garcinia mangostana Linn extract's oral administration. Fifty BALB/c mice were divided into five groups, including doses of 0.5 gram/kg, 1.0 gram/kg, 2.0 gram/kg, negative control, and normal group. Each rat was given an extract by force-feeding via intragastric tube for 14 days (subacute). The results showed that differences in SGOT level (p = 0.061), SGPT level (p = 0.82), and weight gain (p = 0.076) were not significant between the treatment and control groups, accompanied by the absence of clinical signs during the study. These indicate that subacute administration of chitosan-alginate coated microparticle of Garcinia mangostana Linn extract is safe until a dose of 2.0 gram/kgBW. Further studies are needed to evaluate the toxicity effects of this extract on other parameters and in a longer duration of exposure.
KW - chitosan-alginate
KW - Garcinia mangostana Linn
KW - microparticle
KW - subacute toxicity
UR - http://www.scopus.com/inward/record.url?scp=85096440310&partnerID=8YFLogxK
U2 - 10.1063/1.5139326
DO - 10.1063/1.5139326
M3 - Conference contribution
AN - SCOPUS:85076780155
T3 - AIP Conference Proceedings
BT - Recent Progress on
A2 - Yuliusman, Yuliusman
A2 - Dianita, Cindy
PB - American Institute of Physics Inc.
T2 - 16th International Conference on Quality in Research, QiR 2019 - 2019 International Symposium on Sustainable and Clean Energy, ISSCE 2019
Y2 - 22 July 2019 through 24 July 2019
ER -