Chitosan-alginate coated microparticle of Garcinia mangostana Linn extract promises an alternative treatment of colon cancer patients, as the component of Garcinia mangostana Linn extract has anticancer activity in colon cancer cell lines, and chitosan-alginate could deliver compound faster to colon and accelerate its absorption. However, there have not been any studies evaluating sub-acutely the toxicity effects of this compound on the liver (SGOT and SGPT) and clinical signs of toxicity. This study was conducted to evaluate the subacute toxicity effect of Chitosan-alginate coated microparticle of Garcinia mangostana Linn extract's oral administration. Fifty BALB/c mice were divided into five groups, including doses of 0.5 gram/kg, 1.0 gram/kg, 2.0 gram/kg, negative control, and normal group. Each rat was given an extract by force-feeding via intragastric tube for 14 days (subacute). The results showed that differences in SGOT level (p = 0.061), SGPT level (p = 0.82), and weight gain (p = 0.076) were not significant between the treatment and control groups, accompanied by the absence of clinical signs during the study. These indicate that subacute administration of chitosan-alginate coated microparticle of Garcinia mangostana Linn extract is safe until a dose of 2.0 gram/kgBW. Further studies are needed to evaluate the toxicity effects of this extract on other parameters and in a longer duration of exposure.