This research was conducted to study the ability of bisphenol A as a prooxidant. The formation of DNA adduct 8-OHdG was done by reacting dG with bisphenol A with the addition of Fenton's Reagent. DNA adduct 8-OHdG was analyzed by using a reverse phase HPLC with UV/vis detector at 254 nm. The optimum condition to analyze 8-OHdG was obtained by using an eluent with a mixture of phosphate buffer pH 6.7,10 mM and methanol at the ratio of 85:15. The results of this study indicated that bisphenol A act as prooxidant because of the increase yield of 8-OHdG formed in the reaction by the addition of bisphenol A. The addition of Fenton's Reagent also increased the yield of 8-OHdG. Variations in this present study included the variations of temperature, pH, and incubation time. The variations were pH 7.4 and 8.4, temperature of 37°C and 60°C, also the incubation time 7 and 12 hours. Mostly, the concentration of 8-OHdG will increase with the increase of pH, temperature, and with a longer incubation time. In the presence of BPA and Fenton's reagent at pH 7.4, temperature 60°C and 12 hours incubation time, the highest 8-OHdG concentration of 42.258 ppb was detected.