@inproceedings{5dbd16a790d04daeb1d2b26262d56c04,
title = "Study on the formation of DNA adduct 8-hydroxy-2'-deoxyguanosine (8-OHdG) in vitro with bisphenol a through Fenton reaction",
abstract = "This research was conducted to study the ability of bisphenol A as a prooxidant. The formation of DNA adduct 8-OHdG was done by reacting dG with bisphenol A with the addition of Fenton's Reagent. DNA adduct 8-OHdG was analyzed by using a reverse phase HPLC with UV/vis detector at 254 nm. The optimum condition to analyze 8-OHdG was obtained by using an eluent with a mixture of phosphate buffer pH 6.7,10 mM and methanol at the ratio of 85:15. The results of this study indicated that bisphenol A act as prooxidant because of the increase yield of 8-OHdG formed in the reaction by the addition of bisphenol A. The addition of Fenton's Reagent also increased the yield of 8-OHdG. Variations in this present study included the variations of temperature, pH, and incubation time. The variations were pH 7.4 and 8.4, temperature of 37°C and 60°C, also the incubation time 7 and 12 hours. Mostly, the concentration of 8-OHdG will increase with the increase of pH, temperature, and with a longer incubation time. In the presence of BPA and Fenton's reagent at pH 7.4, temperature 60°C and 12 hours incubation time, the highest 8-OHdG concentration of 42.258 ppb was detected.",
keywords = "8-OHdG, BPA, DNA Adduct, Fenton Reaction, ROS",
author = "B. Budiawan and S. Handayani and Dani, {I. C.} and R. Bakri and P. Juniarti and N. Nahla",
note = "Publisher Copyright: {\textcopyright} 2018 Author(s).; 3rd International Symposium on Current Progress in Mathematics and Sciences 2017, ISCPMS 2017 ; Conference date: 26-07-2017 Through 27-07-2017",
year = "2018",
month = oct,
day = "22",
doi = "10.1063/1.5064053",
language = "English",
series = "AIP Conference Proceedings",
publisher = "American Institute of Physics Inc.",
editor = "Ratna Yuniati and Terry Mart and Anggraningrum, {Ivandini T.} and Djoko Triyono and Sugeng, {Kiki A.}",
booktitle = "Proceedings of the 3rd International Symposium on Current Progress in Mathematics and Sciences 2017, ISCPMS 2017",
address = "United States",
}