Study on the formation of DNA adduct 8-hydroxy-2'-deoxyguanosine (8-OHdG) in vitro with bisphenol a through Fenton reaction

Budiawan, Sri Handayani, I. C. Dani, Ridla Bakri, P. Juniarti, N. Nahla

Research output: Chapter in Book/Report/Conference proceedingConference contributionpeer-review

Abstract

This research was conducted to study the ability of bisphenol A as a prooxidant. The formation of DNA adduct 8-OHdG was done by reacting dG with bisphenol A with the addition of Fenton's Reagent. DNA adduct 8-OHdG was analyzed by using a reverse phase HPLC with UV/vis detector at 254 nm. The optimum condition to analyze 8-OHdG was obtained by using an eluent with a mixture of phosphate buffer pH 6.7,10 mM and methanol at the ratio of 85:15. The results of this study indicated that bisphenol A act as prooxidant because of the increase yield of 8-OHdG formed in the reaction by the addition of bisphenol A. The addition of Fenton's Reagent also increased the yield of 8-OHdG. Variations in this present study included the variations of temperature, pH, and incubation time. The variations were pH 7.4 and 8.4, temperature of 37°C and 60°C, also the incubation time 7 and 12 hours. Mostly, the concentration of 8-OHdG will increase with the increase of pH, temperature, and with a longer incubation time. In the presence of BPA and Fenton's reagent at pH 7.4, temperature 60°C and 12 hours incubation time, the highest 8-OHdG concentration of 42.258 ppb was detected.

Original languageEnglish
Title of host publicationProceedings of the 3rd International Symposium on Current Progress in Mathematics and Sciences 2017, ISCPMS 2017
EditorsRatna Yuniati, Terry Mart, Ivandini T. Anggraningrum, Djoko Triyono, Kiki A. Sugeng
PublisherAmerican Institute of Physics Inc.
ISBN (Electronic)9780735417410
DOIs
Publication statusPublished - 22 Oct 2018
Event3rd International Symposium on Current Progress in Mathematics and Sciences 2017, ISCPMS 2017 - Bali, Indonesia
Duration: 26 Jul 201727 Jul 2017

Publication series

NameAIP Conference Proceedings
Volume2023
ISSN (Print)0094-243X
ISSN (Electronic)1551-7616

Conference

Conference3rd International Symposium on Current Progress in Mathematics and Sciences 2017, ISCPMS 2017
CountryIndonesia
CityBali
Period26/07/1727/07/17

Keywords

  • 8-OHdG
  • BPA
  • DNA Adduct
  • Fenton Reaction
  • ROS

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