Study of the toxic effects (synergistic or antagonistic) of bisphenol A and methylparaben on the formation of DNA-adduct (8-OHdG)

R. Debora; B. Budiawan; S. Handayani; I. C. Dani

doi:10.1063/5.0061149

Study of the toxic effects (synergistic or antagonistic) of bisphenol A and methylparaben on the formation of DNA-adduct (8-OHdG)

R. Debora, B. Budiawan, S. Handayani, I. C. Dani

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › peer-review

Abstract

Bisphenol A (BPA) is a chemical that is commonly applied in the manufacture of polycarbonate plastic and epoxy resins which are often used in food and beverage containers. Methylparaben (MeP) is commonly applied as food preservatives, products of personal cares, and medicines. This study was conducted to define the BPA and MeP effects on the formation of DNA-Adduct 8-Hydroxy-2'deoxyguanosine (8-OHdG) compounds. Analysis of 8-OHdG compounds was performed by applying a reverse-phase HPLC with a UV/Vis detector at the wavelength of 254nm. The optimum condition of HPLC was obtained with the mobile phase consisted of phosphate buffer solution and methanol at a ratio of 85:15 (v/v) and a flow rate of 1.2 mL/min. This research was conducted at pH 7.4 and temperature 37 °C with a variation of incubation time of 5 and 7 h. In this study, it was found that the presence of BPA and MeP in the mixtures produced an antagonistic effect to the formation of 8-OHdG compared to BPA and MeP in the separate reaction at the same condition of incubations.

Original language	English
Title of host publication	Proceedings of the 6th International Symposium on Current Progress in Mathematics and Sciences 2020, ISCPMS 2020
Editors	Tribidasari A. Ivandini, David G. Churchill, Youngil Lee, Yatimah Binti Alias, Chris Margules
Publisher	American Institute of Physics Inc.
ISBN (Electronic)	9780735441132
DOIs	https://doi.org/10.1063/5.0061149
Publication status	Published - 23 Jul 2021
Event	6th International Symposium on Current Progress in Mathematics and Sciences 2020, ISCPMS 2020 - Depok, Indonesia Duration: 27 Oct 2020 → 28 Oct 2020

Publication series

Name	AIP Conference Proceedings
Volume	2374
ISSN (Print)	0094-243X
ISSN (Electronic)	1551-7616

Conference

Conference	6th International Symposium on Current Progress in Mathematics and Sciences 2020, ISCPMS 2020
Country/Territory	Indonesia
City	Depok
Period	27/10/20 → 28/10/20

Keywords

8-hydroxy-2' deoxyguanosine
bisphenol A
DNA adduct
methylparaben

Access to Document

10.1063/5.0061149

Cite this

Debora, R., Budiawan, B., Handayani, S., & Dani, I. C. (2021). Study of the toxic effects (synergistic or antagonistic) of bisphenol A and methylparaben on the formation of DNA-adduct (8-OHdG). In T. A. Ivandini, D. G. Churchill, Y. Lee, Y. B. Alias, & C. Margules (Eds.), Proceedings of the 6th International Symposium on Current Progress in Mathematics and Sciences 2020, ISCPMS 2020 Article 040005 (AIP Conference Proceedings; Vol. 2374). American Institute of Physics Inc.. https://doi.org/10.1063/5.0061149

Debora, R. ; Budiawan, B. ; Handayani, S. et al. / Study of the toxic effects (synergistic or antagonistic) of bisphenol A and methylparaben on the formation of DNA-adduct (8-OHdG). Proceedings of the 6th International Symposium on Current Progress in Mathematics and Sciences 2020, ISCPMS 2020. editor / Tribidasari A. Ivandini ; David G. Churchill ; Youngil Lee ; Yatimah Binti Alias ; Chris Margules. American Institute of Physics Inc., 2021. (AIP Conference Proceedings).

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title = "Study of the toxic effects (synergistic or antagonistic) of bisphenol A and methylparaben on the formation of DNA-adduct (8-OHdG)",

abstract = "Bisphenol A (BPA) is a chemical that is commonly applied in the manufacture of polycarbonate plastic and epoxy resins which are often used in food and beverage containers. Methylparaben (MeP) is commonly applied as food preservatives, products of personal cares, and medicines. This study was conducted to define the BPA and MeP effects on the formation of DNA-Adduct 8-Hydroxy-2'deoxyguanosine (8-OHdG) compounds. Analysis of 8-OHdG compounds was performed by applying a reverse-phase HPLC with a UV/Vis detector at the wavelength of 254nm. The optimum condition of HPLC was obtained with the mobile phase consisted of phosphate buffer solution and methanol at a ratio of 85:15 (v/v) and a flow rate of 1.2 mL/min. This research was conducted at pH 7.4 and temperature 37 °C with a variation of incubation time of 5 and 7 h. In this study, it was found that the presence of BPA and MeP in the mixtures produced an antagonistic effect to the formation of 8-OHdG compared to BPA and MeP in the separate reaction at the same condition of incubations.",

keywords = "8-hydroxy-2' deoxyguanosine, bisphenol A, DNA adduct, methylparaben",

author = "R. Debora and B. Budiawan and S. Handayani and Dani, {I. C.}",

note = "Funding Information: The authors are thank to PUTI Prosiding Universitas Indonesia Grant with contract number NKB-965/UN2.RST/HKP.05.00/2020 for funding this research. Publisher Copyright: {\textcopyright} 2021 Author(s).; 6th International Symposium on Current Progress in Mathematics and Sciences 2020, ISCPMS 2020 ; Conference date: 27-10-2020 Through 28-10-2020",

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Debora, R, Budiawan, B , Handayani, S & Dani, IC 2021, Study of the toxic effects (synergistic or antagonistic) of bisphenol A and methylparaben on the formation of DNA-adduct (8-OHdG). in TA Ivandini, DG Churchill, Y Lee, YB Alias & C Margules (eds), Proceedings of the 6th International Symposium on Current Progress in Mathematics and Sciences 2020, ISCPMS 2020., 040005, AIP Conference Proceedings, vol. 2374, American Institute of Physics Inc., 6th International Symposium on Current Progress in Mathematics and Sciences 2020, ISCPMS 2020, Depok, Indonesia, 27/10/20. https://doi.org/10.1063/5.0061149

Study of the toxic effects (synergistic or antagonistic) of bisphenol A and methylparaben on the formation of DNA-adduct (8-OHdG). / Debora, R.; Budiawan, B.; Handayani, S. et al.
Proceedings of the 6th International Symposium on Current Progress in Mathematics and Sciences 2020, ISCPMS 2020. ed. / Tribidasari A. Ivandini; David G. Churchill; Youngil Lee; Yatimah Binti Alias; Chris Margules. American Institute of Physics Inc., 2021. 040005 (AIP Conference Proceedings; Vol. 2374).

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › peer-review

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T1 - Study of the toxic effects (synergistic or antagonistic) of bisphenol A and methylparaben on the formation of DNA-adduct (8-OHdG)

AU - Debora, R.

AU - Budiawan, B.

AU - Handayani, S.

AU - Dani, I. C.

N1 - Funding Information: The authors are thank to PUTI Prosiding Universitas Indonesia Grant with contract number NKB-965/UN2.RST/HKP.05.00/2020 for funding this research. Publisher Copyright: © 2021 Author(s).

PY - 2021/7/23

Y1 - 2021/7/23

N2 - Bisphenol A (BPA) is a chemical that is commonly applied in the manufacture of polycarbonate plastic and epoxy resins which are often used in food and beverage containers. Methylparaben (MeP) is commonly applied as food preservatives, products of personal cares, and medicines. This study was conducted to define the BPA and MeP effects on the formation of DNA-Adduct 8-Hydroxy-2'deoxyguanosine (8-OHdG) compounds. Analysis of 8-OHdG compounds was performed by applying a reverse-phase HPLC with a UV/Vis detector at the wavelength of 254nm. The optimum condition of HPLC was obtained with the mobile phase consisted of phosphate buffer solution and methanol at a ratio of 85:15 (v/v) and a flow rate of 1.2 mL/min. This research was conducted at pH 7.4 and temperature 37 °C with a variation of incubation time of 5 and 7 h. In this study, it was found that the presence of BPA and MeP in the mixtures produced an antagonistic effect to the formation of 8-OHdG compared to BPA and MeP in the separate reaction at the same condition of incubations.

AB - Bisphenol A (BPA) is a chemical that is commonly applied in the manufacture of polycarbonate plastic and epoxy resins which are often used in food and beverage containers. Methylparaben (MeP) is commonly applied as food preservatives, products of personal cares, and medicines. This study was conducted to define the BPA and MeP effects on the formation of DNA-Adduct 8-Hydroxy-2'deoxyguanosine (8-OHdG) compounds. Analysis of 8-OHdG compounds was performed by applying a reverse-phase HPLC with a UV/Vis detector at the wavelength of 254nm. The optimum condition of HPLC was obtained with the mobile phase consisted of phosphate buffer solution and methanol at a ratio of 85:15 (v/v) and a flow rate of 1.2 mL/min. This research was conducted at pH 7.4 and temperature 37 °C with a variation of incubation time of 5 and 7 h. In this study, it was found that the presence of BPA and MeP in the mixtures produced an antagonistic effect to the formation of 8-OHdG compared to BPA and MeP in the separate reaction at the same condition of incubations.

KW - 8-hydroxy-2' deoxyguanosine

KW - bisphenol A

KW - DNA adduct

KW - methylparaben

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M3 - Conference contribution

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A2 - Churchill, David G.

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A2 - Alias, Yatimah Binti

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Debora R, Budiawan B , Handayani S, Dani IC. Study of the toxic effects (synergistic or antagonistic) of bisphenol A and methylparaben on the formation of DNA-adduct (8-OHdG). In Ivandini TA, Churchill DG, Lee Y, Alias YB, Margules C, editors, Proceedings of the 6th International Symposium on Current Progress in Mathematics and Sciences 2020, ISCPMS 2020. American Institute of Physics Inc. 2021. 040005. (AIP Conference Proceedings). doi: 10.1063/5.0061149

Study of the toxic effects (synergistic or antagonistic) of bisphenol A and methylparaben on the formation of DNA-adduct (8-OHdG)

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Keywords

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