TY - GEN
T1 - Study of Majalaya carp (Cyprinus carpio Linn.) chromosome using chromosome image analyzing system
AU - Jesaya, J.
AU - Aundi, S. H.M.
AU - Wardhana, W.
AU - Dwiranti, A.
N1 - Funding Information:
We thank the Directorate of Research and Community Services, Universitas Indonesia (DRPM UI), for allowing us to use the Integrated Laboratory and Research Center (ILRC) during sample preparation. This work is supported by PITTA B Grant 2019 funded by DRPM Universitas Indonesia No. NKB-0615/UN2.R3.1/HKP.05.00/2019 to AD.
Publisher Copyright:
© 2020 Author(s).
PY - 2020/6/1
Y1 - 2020/6/1
N2 - Majalaya carp (Cyprinus carpio L.) is a widely spread strain of common carp in Indonesia. Although it is well-known and thus the cultivation data is comprehensive, the cytogenetical information has yet to be completed. This research aimed to analyze the chromosome number and size of the Majalaya carp from gills. Furthermore, the small size and the great number of C. carpio L. chromosome has been a problem to be solved in the chromosomal study. Thus, this study applied the Chromosome Image Analyzing System (CHIAS) for the first time to analyze the chromosome of Majalaya carp in Indonesia. Chromosomes were taken from the gills. Gills were subjected to the synchronization steps using colcemid, homogenized, hydrolyzed, fixed, and finally were stained by using Giemsa. The results showed that the number of Majalaya carp chromosomes were 2n=100∼102. In addition, the average length of the Majalaya carp chromosome (C. carpio) was 0.725 ± 0.287 μ m and the average width was 0.756 ± 0.297 μ m. Furthermore, by using CHIAS, the small size of the chromosome could still be arranged, suggesting the advantages of CHIAS for chromosome analysis. The chromosome information obtained in this study could be used as the basic data for further chromosome analysis. This study also suggested the application of CHIAS for chromosomal analysis of any other species.
AB - Majalaya carp (Cyprinus carpio L.) is a widely spread strain of common carp in Indonesia. Although it is well-known and thus the cultivation data is comprehensive, the cytogenetical information has yet to be completed. This research aimed to analyze the chromosome number and size of the Majalaya carp from gills. Furthermore, the small size and the great number of C. carpio L. chromosome has been a problem to be solved in the chromosomal study. Thus, this study applied the Chromosome Image Analyzing System (CHIAS) for the first time to analyze the chromosome of Majalaya carp in Indonesia. Chromosomes were taken from the gills. Gills were subjected to the synchronization steps using colcemid, homogenized, hydrolyzed, fixed, and finally were stained by using Giemsa. The results showed that the number of Majalaya carp chromosomes were 2n=100∼102. In addition, the average length of the Majalaya carp chromosome (C. carpio) was 0.725 ± 0.287 μ m and the average width was 0.756 ± 0.297 μ m. Furthermore, by using CHIAS, the small size of the chromosome could still be arranged, suggesting the advantages of CHIAS for chromosome analysis. The chromosome information obtained in this study could be used as the basic data for further chromosome analysis. This study also suggested the application of CHIAS for chromosomal analysis of any other species.
KW - CHIAS
KW - Chromosome
KW - Cyprinus carpio
KW - Majalaya carp
UR - http://www.scopus.com/inward/record.url?scp=85086656493&partnerID=8YFLogxK
U2 - 10.1063/5.0008275
DO - 10.1063/5.0008275
M3 - Conference contribution
AN - SCOPUS:85086656493
T3 - AIP Conference Proceedings
BT - Proceedings of the 5th International Symposium on Current Progress in Mathematics and Sciences, ISCPMS 2019
A2 - Mart, Terry
A2 - Triyono, Djoko
A2 - Ivandini, Tribidasari Anggraningrum
PB - American Institute of Physics Inc.
T2 - 5th International Symposium on Current Progress in Mathematics and Sciences, ISCPMS 2019
Y2 - 9 July 2019 through 10 July 2019
ER -