Majalaya carp (Cyprinus carpio L.) is a widely spread strain of common carp in Indonesia. Although it is well-known and thus the cultivation data is comprehensive, the cytogenetical information has yet to be completed. This research aimed to analyze the chromosome number and size of the Majalaya carp from gills. Furthermore, the small size and the great number of C. carpio L. chromosome has been a problem to be solved in the chromosomal study. Thus, this study applied the Chromosome Image Analyzing System (CHIAS) for the first time to analyze the chromosome of Majalaya carp in Indonesia. Chromosomes were taken from the gills. Gills were subjected to the synchronization steps using colcemid, homogenized, hydrolyzed, fixed, and finally were stained by using Giemsa. The results showed that the number of Majalaya carp chromosomes were 2n=100∼102. In addition, the average length of the Majalaya carp chromosome (C. carpio) was 0.725 ± 0.287 μ m and the average width was 0.756 ± 0.297 μ m. Furthermore, by using CHIAS, the small size of the chromosome could still be arranged, suggesting the advantages of CHIAS for chromosome analysis. The chromosome information obtained in this study could be used as the basic data for further chromosome analysis. This study also suggested the application of CHIAS for chromosomal analysis of any other species.