The study of heavy metal depuration, Nickel (Ni) and Chromium (Cr), in marine species (Perna viridis) from Kamal Estuary, North Jakarta was observed in this research. The concentrations of Ni and Cr in Perna viridis from Kamal Estuary were determined as control. The depuration was conducted with acetic acid in various concentrations 1%, 3%, 5%, and 10%, and water temperature variations of 60°C, 80°C, and 100°C. Both acetic acid and water depuration treatments were obtained at 1, 2, and 4 hours immersion, with and without stirring. In the control groups, Ni metal content is obtained as much as 2.113 mg/kg and for Cr is 3.426 mg/kg. The greatest decrease in metal content was obtained by the immersion treatment of 10% acetic acid for 4 hour with stirring, which are 0.085 mg/kg for Ni and 1.492 mg/kg for Cr after treatment. Protein content was also determined before and after depuration by Kjeldahl method to investigate the possibility of damage and loss of amino acids. Protein content before depuration is 20.544% and for treatment after depuration with the greatest decrease in metal content is 20.475%.