Study of DNA adduct 8 hydroxy-2'-deoxyguanosine (8-OHdG) formation through fenton reaction with tert-butylhydroquinone (TBHQ) and butyl hydroxy toluene (BHT)

Sri Handayani, I. C. Dani, Budiawan, D. Pakuanisa

Research output: Contribution to journalConference articlepeer-review

1 Citation (Scopus)

Abstract

The research of DNA adduct formation 8-hydroxy-2'-Deoxyguanosine (8-OHdG) as a biomarker of DNA damage due to oxidative stress was carried out by reacting the DNA base 2'-deoxyguanosine-5'-monophosphate with TBHQ and BHT. The formationof 8-OHdG was carried out in various conditions, at temperature of 37° C and 60° C, pH 7.4 and pH 8.4, within 5 hours of incubation time and in the addition of FeSO 4 . The formation of DNA adducts profile were analyzed using reversed phase HPLC with UV detector at a wavelength of 254 nm. The results of the study showed that TBHQ and BHT can trigger the formation of 8-OHdG from the reaction of 2'-hydroxy Deoxyguanosine-5'-monophosphate in the presence of Fe (II). Meanwhile, in the addition of hydrogen peroxide, the formation of DNA adducts only occur in the test substance TBHQ. The results showed that the condition of higher temperature at 60°C and pH 8,4 affects the higher formation of DNA adducts.

Original languageEnglish
Article number012015
JournalJournal of Physics: Conference Series
Volume835
Issue number1
DOIs
Publication statusPublished - 17 Apr 2017
EventInternational Symposium on Bioinformatics, Chemometrics and Metabolomics, ISBCM 2016 - Bogor, Indonesia
Duration: 18 Oct 2016 → …

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