Study of alpha (α)-tocopherol antioxidant towards garut sheeps (Ovis aries) oocyte maturation and viability post-cryopreservation using slow freezing method

M. Gunawan, M. R. Pahlevi, E. M. Kaiin, A. Bowolaksono

Research output: Chapter in Book/Report/Conference proceedingConference contributionpeer-review

Abstract

The study of the potential alpha (α)-tocopherol antioxidants towards garut sheep (Ovis aries) oocyte maturation and viability post-cryopreservation using slow freezing method has been studied. This study aimed to determine and evaluate the optimal concentration of adding alpha (α)-tocopherol towards oocyte in vitro maturation and viability post-cryopreservation. A total of 138 oocytes which have A and B qualities, were matured in TCM-199 medium with the addition of alpha (α)-tocopherol antioxidants at concentrations of 0 μM, 100 μM, 150 μM, and 200 μM. Oocytes from in vitro maturation are then cryopreserved and the maturity and viability was observed. Observation of oocyte maturation was based on the formation of the polar body I. Observation of oocytes viability used Hoechst and propidium iodide dyes. This experiment showed that there was no significant (P > 0.05) difference between concentrations, but the results of developments in concentration of 150 μM have the highest percentage of oocyte maturation (88.57 %) and viability (82.86 %). In conclusion, the presence of 150 μM alpha (α)-tocopherol was the optimal concentration that is able to maintain the oocyte membrane stability post-cryopreservation.

Original languageEnglish
Title of host publicationProceedings of the 5th International Symposium on Current Progress in Mathematics and Sciences, ISCPMS 2019
EditorsTerry Mart, Djoko Triyono, Tribidasari Anggraningrum Ivandini
PublisherAmerican Institute of Physics Inc.
ISBN (Electronic)9780735420014
DOIs
Publication statusPublished - 1 Jun 2020
Event5th International Symposium on Current Progress in Mathematics and Sciences, ISCPMS 2019 - Depok, Indonesia
Duration: 9 Jul 201910 Jul 2019

Publication series

NameAIP Conference Proceedings
Volume2242
ISSN (Print)0094-243X
ISSN (Electronic)1551-7616

Conference

Conference5th International Symposium on Current Progress in Mathematics and Sciences, ISCPMS 2019
CountryIndonesia
CityDepok
Period9/07/1910/07/19

Keywords

  • Alpha (α)-tocopherol
  • in vitro maturation
  • oocyte
  • viability

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