Study of 8-OHdG formation through incubation of base nucleotides DNA 2'-deoxyguanosine on Bisphenol A (BPA) and Ni (II)

A. Rahmadewi; I. C. Dani

doi:10.1063/5.0001085

Study of 8-OHdG formation through incubation of base nucleotides DNA 2'-deoxyguanosine on Bisphenol A (BPA) and Ni (II)

A. Rahmadewi, Budiawan, I. C. Dani

Department of Chemistry

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › peer-review

Abstract

Bisphenol A (BPA) is an industrial chemical used in food contact materials such as canned coatings. Meanwhile Ni (II) is one of the metal compounds that can be found naturally in food and water, and might be increased due to industrial waste. Both of BPA and Ni (II) are categorized as carcinogenic compounds that have possibility to trigger cancer in human. This study was conducted to analyze the effects of BPA and Ni (II) exposure on the formation of DNA adduct 8-hydroxy-2'-deoxyguanosine (8-OHdG) as biomarker of risk cancer through in vitro reaction using 2'-deoxyguanosine. Our previous in vitro studies were conducted in physiological condition, while in this study we traced the formation of 8-OHdG by reacting 2'-deoxyguanosine with various mixture of BPA and Ni (II) in the presence of H₂O₂ as oxidizing agent at pH 8.4, temperature 60 °C, and two incubation time 7 and 12 hours. Analysis of 8-OHdG formation was carried out by using a UHPLC reverse phase with UV-Vis detector at 254 nm and C18 as a stationary phase. The results showed that the highest concentration of 8-OHdG was on samples that contains BPA and H₂O₂ with 12 hours incubation time, as much as 85.313 ppb.

Original language	English
Title of host publication	8th International Conference of the Indonesian Chemical Society, ICICS 2019
Editors	Mohamad Rafi, Rudi Heryanto, Wulan Tri Wahyuni
Publisher	American Institute of Physics Inc.
ISBN (Electronic)	9780735420021
DOIs	https://doi.org/10.1063/5.0001085
Publication status	Published - 4 Jun 2020
Event	8th International Conference of the Indonesian Chemical Society 2019, ICICS 2019 - Bogor, Indonesia Duration: 6 Aug 2019 → 7 Aug 2019

Publication series

Name	AIP Conference Proceedings
Volume	2243
ISSN (Print)	0094-243X
ISSN (Electronic)	1551-7616

Conference

Conference	8th International Conference of the Indonesian Chemical Society 2019, ICICS 2019
Country/Territory	Indonesia
City	Bogor
Period	6/08/19 → 7/08/19

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1063/5.0001085

Cite this

Rahmadewi, A., Budiawan, & Dani, I. C. (2020). Study of 8-OHdG formation through incubation of base nucleotides DNA 2'-deoxyguanosine on Bisphenol A (BPA) and Ni (II). In M. Rafi, R. Heryanto, & W. T. Wahyuni (Eds.), 8th International Conference of the Indonesian Chemical Society, ICICS 2019 Article 020020 (AIP Conference Proceedings; Vol. 2243). American Institute of Physics Inc.. https://doi.org/10.1063/5.0001085

Rahmadewi, A. ; Budiawan ; Dani, I. C. / Study of 8-OHdG formation through incubation of base nucleotides DNA 2'-deoxyguanosine on Bisphenol A (BPA) and Ni (II). 8th International Conference of the Indonesian Chemical Society, ICICS 2019. editor / Mohamad Rafi ; Rudi Heryanto ; Wulan Tri Wahyuni. American Institute of Physics Inc., 2020. (AIP Conference Proceedings).

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title = "Study of 8-OHdG formation through incubation of base nucleotides DNA 2'-deoxyguanosine on Bisphenol A (BPA) and Ni (II)",

abstract = "Bisphenol A (BPA) is an industrial chemical used in food contact materials such as canned coatings. Meanwhile Ni (II) is one of the metal compounds that can be found naturally in food and water, and might be increased due to industrial waste. Both of BPA and Ni (II) are categorized as carcinogenic compounds that have possibility to trigger cancer in human. This study was conducted to analyze the effects of BPA and Ni (II) exposure on the formation of DNA adduct 8-hydroxy-2'-deoxyguanosine (8-OHdG) as biomarker of risk cancer through in vitro reaction using 2'-deoxyguanosine. Our previous in vitro studies were conducted in physiological condition, while in this study we traced the formation of 8-OHdG by reacting 2'-deoxyguanosine with various mixture of BPA and Ni (II) in the presence of H2O2 as oxidizing agent at pH 8.4, temperature 60 °C, and two incubation time 7 and 12 hours. Analysis of 8-OHdG formation was carried out by using a UHPLC reverse phase with UV-Vis detector at 254 nm and C18 as a stationary phase. The results showed that the highest concentration of 8-OHdG was on samples that contains BPA and H2O2 with 12 hours incubation time, as much as 85.313 ppb.",

author = "A. Rahmadewi and Budiawan and Dani, {I. C.}",

note = "Funding Information: We are thanks to Hibah PITTA B DRPM UI with contract number 476/SK/R/UI/2019 for funding this research and Department of Chemistry for providing the UHPLC instrument. Publisher Copyright: {\textcopyright} 2020 Author(s).; 8th International Conference of the Indonesian Chemical Society 2019, ICICS 2019 ; Conference date: 06-08-2019 Through 07-08-2019",

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Rahmadewi, A, Budiawan & Dani, IC 2020, Study of 8-OHdG formation through incubation of base nucleotides DNA 2'-deoxyguanosine on Bisphenol A (BPA) and Ni (II). in M Rafi, R Heryanto & WT Wahyuni (eds), 8th International Conference of the Indonesian Chemical Society, ICICS 2019., 020020, AIP Conference Proceedings, vol. 2243, American Institute of Physics Inc., 8th International Conference of the Indonesian Chemical Society 2019, ICICS 2019, Bogor, Indonesia, 6/08/19. https://doi.org/10.1063/5.0001085

Study of 8-OHdG formation through incubation of base nucleotides DNA 2'-deoxyguanosine on Bisphenol A (BPA) and Ni (II). / Rahmadewi, A.; Budiawan; Dani, I. C.
8th International Conference of the Indonesian Chemical Society, ICICS 2019. ed. / Mohamad Rafi; Rudi Heryanto; Wulan Tri Wahyuni. American Institute of Physics Inc., 2020. 020020 (AIP Conference Proceedings; Vol. 2243).

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › peer-review

TY - GEN

T1 - Study of 8-OHdG formation through incubation of base nucleotides DNA 2'-deoxyguanosine on Bisphenol A (BPA) and Ni (II)

AU - Rahmadewi, A.

AU - Budiawan, null

AU - Dani, I. C.

N1 - Funding Information: We are thanks to Hibah PITTA B DRPM UI with contract number 476/SK/R/UI/2019 for funding this research and Department of Chemistry for providing the UHPLC instrument. Publisher Copyright: © 2020 Author(s).

PY - 2020/6/4

Y1 - 2020/6/4

N2 - Bisphenol A (BPA) is an industrial chemical used in food contact materials such as canned coatings. Meanwhile Ni (II) is one of the metal compounds that can be found naturally in food and water, and might be increased due to industrial waste. Both of BPA and Ni (II) are categorized as carcinogenic compounds that have possibility to trigger cancer in human. This study was conducted to analyze the effects of BPA and Ni (II) exposure on the formation of DNA adduct 8-hydroxy-2'-deoxyguanosine (8-OHdG) as biomarker of risk cancer through in vitro reaction using 2'-deoxyguanosine. Our previous in vitro studies were conducted in physiological condition, while in this study we traced the formation of 8-OHdG by reacting 2'-deoxyguanosine with various mixture of BPA and Ni (II) in the presence of H2O2 as oxidizing agent at pH 8.4, temperature 60 °C, and two incubation time 7 and 12 hours. Analysis of 8-OHdG formation was carried out by using a UHPLC reverse phase with UV-Vis detector at 254 nm and C18 as a stationary phase. The results showed that the highest concentration of 8-OHdG was on samples that contains BPA and H2O2 with 12 hours incubation time, as much as 85.313 ppb.

AB - Bisphenol A (BPA) is an industrial chemical used in food contact materials such as canned coatings. Meanwhile Ni (II) is one of the metal compounds that can be found naturally in food and water, and might be increased due to industrial waste. Both of BPA and Ni (II) are categorized as carcinogenic compounds that have possibility to trigger cancer in human. This study was conducted to analyze the effects of BPA and Ni (II) exposure on the formation of DNA adduct 8-hydroxy-2'-deoxyguanosine (8-OHdG) as biomarker of risk cancer through in vitro reaction using 2'-deoxyguanosine. Our previous in vitro studies were conducted in physiological condition, while in this study we traced the formation of 8-OHdG by reacting 2'-deoxyguanosine with various mixture of BPA and Ni (II) in the presence of H2O2 as oxidizing agent at pH 8.4, temperature 60 °C, and two incubation time 7 and 12 hours. Analysis of 8-OHdG formation was carried out by using a UHPLC reverse phase with UV-Vis detector at 254 nm and C18 as a stationary phase. The results showed that the highest concentration of 8-OHdG was on samples that contains BPA and H2O2 with 12 hours incubation time, as much as 85.313 ppb.

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U2 - 10.1063/5.0001085

DO - 10.1063/5.0001085

M3 - Conference contribution

AN - SCOPUS:85086646819

T3 - AIP Conference Proceedings

BT - 8th International Conference of the Indonesian Chemical Society, ICICS 2019

A2 - Rafi, Mohamad

A2 - Heryanto, Rudi

A2 - Wahyuni, Wulan Tri

PB - American Institute of Physics Inc.

T2 - 8th International Conference of the Indonesian Chemical Society 2019, ICICS 2019

Y2 - 6 August 2019 through 7 August 2019

ER -

Rahmadewi A, Budiawan, Dani IC. Study of 8-OHdG formation through incubation of base nucleotides DNA 2'-deoxyguanosine on Bisphenol A (BPA) and Ni (II). In Rafi M, Heryanto R, Wahyuni WT, editors, 8th International Conference of the Indonesian Chemical Society, ICICS 2019. American Institute of Physics Inc. 2020. 020020. (AIP Conference Proceedings). doi: 10.1063/5.0001085

Study of 8-OHdG formation through incubation of base nucleotides DNA 2'-deoxyguanosine on Bisphenol A (BPA) and Ni (II)

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