STRES OKSDATIF OTAK TIKUS PADA INDUKSI HIPOKSIA SISTEMIK KRONIK

Introduction: Brain is a metabolically active organ which consumes 20% of total oxygen and glucose entering the human body. As a result of high energy metabolism level, brain become more sensitive to oxidative stress. Aims: This study aims to proof the state of oxidative stress, and to analyze the relationship between malonyl dial- dehyde (MDA) and gluthation (GSH) level, with oxidative stress induced by chronic systemic hypoxia (CSH) in rat brain. Methods: An in vivo experimental model of CSH was carried out using rats, which were randomly divided into 6 treatment groups, i.e. group I as a control group or without hypoxic condition, groups II, III, IV, V, and VI were treated under hypoxic condition for 1, 3, 5, 7, and 14 days, respectively. The results were then analyzed using an ANOVA test and Pearson correlation test. Results: Increased levels of MDA highest and lowest GSH levels decrease rat brains hypoxia occurs in 5 days, MDA correlation with GSH obtained strong and significant negative relationship. Discussion: The CSH induction was able to create an oxidative stress conditions that was identified by an increased MDA and decreased GSH rat brains level. on the 5th day. There were strong negative and significant correlation between MDA and GSH level.