Spermatozoa motility of giant grouper (Epinephelus lanceolatus) 48 hours: The role of soybean milk as a cryoprotectant

R. T. Afni, A. Abinawanto, R. Lestari, A. Bowolaksono, N. G. Zavitri

Research output: Chapter in Book/Report/Conference proceedingConference contributionpeer-review

Abstract

The purpose of the study was to investigate the spermatozoa motility of giant grouper (Epinephelus lanceolatus) 48 hours after frozen. The sperm was gathered by hand stripping method and then frozen at -20 °C after diluted by soybean milk. The various amounts of soybean milk used in this experiment were 5 %, 10 %, 15 %, 20 % and 25 %, respectively. Data were analyzed statistically either by ANOVA or Tukey test. The result showed that soybean milk had a significant effect (P < 0.05) in protecting spermatozoa after frozen. The highest percentage of spermatozoa motility (81.17±1.92 %) was shown by 15 % of soybean milk. Therefore, 15 % of soybean milk was the optimum concentration to protect giant grouper spermatozoa.

Original languageEnglish
Title of host publicationProceedings of the 4th International Symposium on Current Progress in Mathematics and Sciences, ISCPMS 2018
EditorsTerry Mart, Djoko Triyono, Ivandini T. Anggraningrum
PublisherAmerican Institute of Physics Inc.
ISBN (Electronic)9780735419155
DOIs
Publication statusPublished - 4 Nov 2019
Event4th International Symposium on Current Progress in Mathematics and Sciences 2018, ISCPMS 2018 - Depok, Indonesia
Duration: 30 Oct 201831 Oct 2018

Publication series

NameAIP Conference Proceedings
Volume2168
ISSN (Print)0094-243X
ISSN (Electronic)1551-7616

Conference

Conference4th International Symposium on Current Progress in Mathematics and Sciences 2018, ISCPMS 2018
CountryIndonesia
CityDepok
Period30/10/1831/10/18

Keywords

  • Cryopreservation
  • Epinephelus lanceolatus
  • soybean milk
  • spermatozoa

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