@inproceedings{c5698e03d5954612b58432833c278b92,
title = "Spermatozoa motility of giant grouper (Epinephelus lanceolatus) 48 hours: The role of soybean milk as a cryoprotectant",
abstract = "The purpose of the study was to investigate the spermatozoa motility of giant grouper (Epinephelus lanceolatus) 48 hours after frozen. The sperm was gathered by hand stripping method and then frozen at -20 °C after diluted by soybean milk. The various amounts of soybean milk used in this experiment were 5 %, 10 %, 15 %, 20 % and 25 %, respectively. Data were analyzed statistically either by ANOVA or Tukey test. The result showed that soybean milk had a significant effect (P < 0.05) in protecting spermatozoa after frozen. The highest percentage of spermatozoa motility (81.17±1.92 %) was shown by 15 % of soybean milk. Therefore, 15 % of soybean milk was the optimum concentration to protect giant grouper spermatozoa.",
keywords = "Cryopreservation, Epinephelus lanceolatus, soybean milk, spermatozoa",
author = "Afni, {R. T.} and A. Abinawanto and R. Lestari and A. Bowolaksono and Zavitri, {N. G.}",
note = "Publisher Copyright: {\textcopyright} 2019 Author(s).; 4th International Symposium on Current Progress in Mathematics and Sciences 2018, ISCPMS 2018 ; Conference date: 30-10-2018 Through 31-10-2018",
year = "2019",
month = nov,
day = "4",
doi = "10.1063/1.5132519",
language = "English",
series = "AIP Conference Proceedings",
publisher = "American Institute of Physics Inc.",
editor = "Terry Mart and Djoko Triyono and Anggraningrum, {Ivandini T.}",
booktitle = "Proceedings of the 4th International Symposium on Current Progress in Mathematics and Sciences, ISCPMS 2018",
address = "United States",
}