Sperm Na + ,K + -ATPase and dynein ATPase activity: A study of embryo development in in vitro fertilization (IVF)

Silvia W. Lestari, Aucky Hinting, Hamdani Lunardi, Debby Aditya, Dessy Noor Miati, Meidika Dara Rizki

Research output: Chapter in Book/Report/Conference proceedingConference contributionpeer-review

Abstract

The published data represented that poor semen parameters resulted in the poor embryo development rate following in vitro Fertilization (IVF) - intracytoplasmic sperm injection (ICSI). The disorder of sperm motility involving Na + ,K + -ATPase and dynein ATPase enzymes disruption may contribute to the paternal effects on embryo quality and development. This study investigated the activities of Na + ,K + -ATPase and dynein ATPase in human sperms of infertile men and the correlation to the ICSI outcome referring to the embryo development. Semen samples were obtained from men performing sperm preparation for the ICSI. The World Health Organization (WHO) 2010 Guidance was used to perform semen analysis. Next, the enzyme activity was measured based on the ability of ATPase to release organic phosphate from ATP as a substrate. The ICSI outcomes including the embryo development grading and scoring were recorded and analyzed. The value of Na + ,K + -ATPase and dynein ATPase were 1.847±0.694 and 1.459±0.843μmol Pi/mg protein/h, while the embryo scoring was 91.83. Using the cut-off value of Na + ,K + -ATPase and dynein ATPase, we found that the low level Na + ,K + -ATPase and dynein ATPase correlated with the low embryo development quality (p<0.05 and p>0.05, respectively). This study demonstrated that poor activities of Na + ,K + -ATPase and dynein ATPase could affect embryo the development following the ICSI.

Original languageEnglish
Title of host publication3rd Biomedical Engineering''s Recent Progress in Biomaterials, Drugs Development, and Medical Devices
Subtitle of host publicationProceedings of the International Symposium of Biomedical Engineering, ISBE 2018
EditorsPraswasti P.D.K. Wulan, Misri Gozan, Sotya Astutiningsih, Ghiska Ramahdita, Radon Dhelika, Prasetyanugraheni Kreshanti
PublisherAmerican Institute of Physics Inc.
ISBN (Electronic)9780735418226
DOIs
Publication statusPublished - 9 Apr 2019
Event3rd International Symposium of Biomedical Engineering''s Recent Progress in Biomaterials, Drugs Development, and Medical Devices, ISBE 2018 - Jakarta, Indonesia
Duration: 6 Aug 20188 Aug 2018

Publication series

NameAIP Conference Proceedings
Volume2092
ISSN (Print)0094-243X
ISSN (Electronic)1551-7616

Conference

Conference3rd International Symposium of Biomedical Engineering''s Recent Progress in Biomaterials, Drugs Development, and Medical Devices, ISBE 2018
CountryIndonesia
CityJakarta
Period6/08/188/08/18

Keywords

  • dynein ATPase
  • embryo development quality
  • Na ,K ATPase

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