The effect of the commercial honey combined with 10% of Dimetyl Sulfoxide (DMSO) to preserve spermatozoa of gourami, Osphronemus goramy for 48 hours at-34°C has been studied. The concentrations of honey used in this study were 0%, 0.1%, 0.3%, 0.5%, 0.7%, and 0.9% (v/v), respectively. The sperms were diluted with the combination of 10% of DMSO, fish ringer and honey (1 part of sample + 3 parts of solvent) and were equilibrated for 10 minutes at 4°C before frozen for 48 hours at-34°C. After freezing, sperms were thawed for 1-2 min at 40°C. Spermatozoa motility was then observed. The ANOVA test showed that honey had a significant effect on the motility rate (p < 0.05). The Tukey multiple-range test revealed that 10% of DMSO combined with 0.7% of honey gave the highest motility (80.48±7.18%). Therefore, it is concluded that 0.7% of honey is the suitable concentration for gourami spermatozoa.
|Number of pages||8|
|Publication status||Published - 2017|
- Commercial honey
- Osphronemus goramy
- Spermatozoa motility