Spectroscopic and physicochemical characteristic of ozonated rice brand oil as antimicrobial

E. Enjarlis, Sofa Fajriah, Setijo Bismo, Dika Hardika, S. R.I. Handayani, Sony Oscar Effendi, Marcelinus Christwardana

Research output: Contribution to journalArticlepeer-review

1 Citation (Scopus)


Rice Brand Oil (RBO) is a vegetable oil, containing active substances that are very good for health and medicine. The use of RBO can be expanded by ozonation of unsaturated fatty acids in RBO to produce 1,2,4 trioxolane, aldehydes, hydroperoxides and peroxides as anti-viruses in patients with HIV, hepatitis, bird flu etc. The effect of the ozonation process on the characteristics of Rice Bran Oil (RBO) is estimated. Spectroscopic characteristics were analysed by GCMS and13C-NMR, while physical and chemical characteristics were studied by density, viscosity, pH, iodine number, peroxide number, and acid number. In addition, RBO color changes during the ozonation process also change. The carbon double bond in the RBO decreases with increasing ozonation time. 1,2,4 Trioxolane, aldehydes and peroxide are the main products in ozonation reactions. The presence of 1,2,4-trioxolane can be removed by gas chromatography and nuclear magnetic resonance spectroscopy analysis, where it arises during the 25-hour ozonation process, and the expanded field increases and ozonates for 175 hours. From13C-NMR spectrum, the presence of ozonides was confirmed by the signals at δC 104.5 ppm. Some saturated fatty acids formed during the ozonation processes from GCMS analysis, such as 1,1-dimethoxydodecane and methyl myristate, proved that ozone as mediator reacted with the unsaturated fatty acid and broke down the double chain of C=C bond to become-C-C-bond. The amount of iodine decreases because the carbon double bonds change into a single bond and the amount of peroxide and acid increases because the component bonds have a single carbon bond, namely 1,2,4 Trioxolane, aldehydes and peroxide. The formation of 1,2,4-trioxolane and other single carbon bond compositions having higher molecular weight increases density and viscosity. Using existing ozone generators requires a longer ozonation time to place the RBO in saturated conditions and make 1, 2, 4-trioxolane unstable and degraded.

Original languageEnglish
Pages (from-to)573-584
Number of pages12
JournalInternational Journal of Pharmaceutical Research
Publication statusPublished - 1 Oct 2019


  • C NMR
  • 1,2,4-Trioxolane
  • Iodine Number
  • Ozone
  • Peroxide Number


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