Spectrophotometric analysis of streptococcus mutans growth and biofilm formation in Saliva and histatin-5 relate to pH and viscosity

Dharli Syafriza, Heriandi Sutadi, Ameta Primasari, Yahwardiah Siregar

Research output: Contribution to journalArticlepeer-review

Abstract

Objective: To analyze the ability of saliva in controlling the growth and the biofilm formation of Streptococcus mutans (S. mutans) as well as the effect of histatin-5 anti-biofilm relate to pH and saliva viscosity. Material and Methods: The S. mutans biofilm assayed by crystal violet 1% and its growth measured by spectrophotometer. The saliva viscosity was analyzed by viscometer, and pH of saliva was measured by pH meter. Results: Based on the optical density values, growth of S. mutans in saliva ranged <300 CFU/mL (0.1 nm) at concentrations of 25%, 12.5% and 6.25% for 24 hours. Whereas at the 48 h and 72 h period of incubation shown an increase in growth of S. mutans ranged 300-600 CFU/mL (0.2-0.36 nm). The inhibitory biofilm formation of S. mutans in saliva was significantly higher at concentrations of 12.5% and 6.25% at 24 h incubation times on a moderate scale, whereas the histatin-5 was effective to inhibit S. mutans biofilm on the 50 and 25 ppm. The saliva possessed a higher inhibitory of biofilm S. mutans than histatin-5 and good level viscosity (0.91-0.92 cP). Conclusion: The saliva was able to control the growth of S. mutans, and histatin-5 can inhibit the biofilm formation S. mutans. Furthermore, the saliva was also able to respond to the pH change with good viscosity of saliva.

Original languageEnglish
Article numbere0018
Pages (from-to)1-11
Number of pages11
JournalPesquisa Brasileira em Odontopediatria e Clinica Integrada
Volume21
DOIs
Publication statusPublished - 2020

Keywords

  • Biofilms
  • Histatins
  • Hydrogen-ion concentration
  • Saliva
  • Viridans streptococci

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