Specificity change of antibody to (4-hydroxy-3-nitrophenyl)acetyl haptens by somatic hypermutation

Amin Subandrio W. Kusumo, Takachika Azuma, Yoshio Hamada, Nobuo Sakato, Hajime Fujio

Research output: Contribution to journalArticlepeer-review

2 Citations (Scopus)


Change in the specificity of anti-(4-hydroxy-3-nitrophenyl)acetyl (NP) antibodies (Abs) with time after immunization was studied. The early anti-NP Abs was specific to the ionized (phenolate) form of NP. The specificity changed with time and the late Abs became able to bind to the protonated (phenolic) form as well as the phenolate form of NP. The nucleotide sequences of mRNA coding for variable regions of heavy and light chains suggested that somatic hypermutation contributed to this change of the specificity.

Original languageEnglish
Pages (from-to)1337-1343
Number of pages7
JournalJournal of Biochemistry
Issue number6
Publication statusPublished - Dec 1987


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