Specificity and sensitivity of a rapid dipstick test (Brugia Rapid) in the detection of Brugia malayi infection

N. Rahmah; S. Taniawati; R. K. Shenoy; B. H. Lim; V. Kumaraswami; A. Khairul Anuar; S. Lokman Hakim; M. I.Noor Hayati; B. T. Chan; M. Suharni; C. P. Ramachandran

doi:10.1016/S0035-9203(01)90091-4

Specificity and sensitivity of a rapid dipstick test (Brugia Rapid) in the detection of Brugia malayi infection

N. Rahmah, S. Taniawati, R. K. Shenoy, B. H. Lim, V. Kumaraswami, A. Khairul Anuar, S. Lokman Hakim, M. I.Noor Hayati, B. T. Chan, M. Suharni, C. P. Ramachandran

Department of Parasitology Pre Clinic

Research output: Contribution to journal › Article › peer-review

62 Citations (Scopus)

Abstract

A total of 753 serum samples from 6 institutions in 3 countries (Malaysia, Indonesia and India) were used to evaluate an immunochromatographic rapid dipstick test, Brugia Rapid, for diagnosis of Brugia malayi infection. The samples comprised sera from 207 microfilaria-positive individuals and 546 individuals from filaria non-endemic areas. The latter consisted of 70 individuals with soil-transmitted helminth infections, 68 with other helminth infections, 238 with protozoan infections, 12 with bacterial and viral infections and 158 healthy individuals. The dipstick is prepared with a goat anti-mouse antibody control line and a B. malayi recombinant-antigen test line. First, the dipstick is dipped into a well containing diluted patient serum, thus allowing specific anti-filarial antibody in the serum to react with the recombinant antigen. Then the dipstick is placed into an adjacent well containing reconstituted anti-human IgG4-gold. After 10 min, development of 2 red-purplish lines denotes a positive result and one line indicates a negative reaction. The overall results of the evaluation showed 97% sensitivity, 99% specificity, 97% positive predictive value and 99% negative predictive value. Brugia Rapid is thus a promising diagnostic tool for detection of B. malayi infection, and would be especially useful for the brugian filariasis elimination programme.

Original language	English
Pages (from-to)	601-604
Number of pages	4
Journal	Transactions of the Royal Society of Tropical Medicine and Hygiene
Volume	95
Issue number	6
DOIs	https://doi.org/10.1016/S0035-9203(01)90091-4
Publication status	Published - 2001

Keywords

Brugia malayi
Diagnosis
Dipstick
Evaluation
Filariasis
IgG4
India
Indonesia
Malaysia
Rapid test
Recombinant antigen

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/S0035-9203(01)90091-4

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Rahmah, N., Taniawati, S., Shenoy, R. K., Lim, B. H., Kumaraswami, V., Anuar, A. K., Hakim, S. L., Hayati, M. I. N., Chan, B. T., Suharni, M., & Ramachandran, C. P. (2001). Specificity and sensitivity of a rapid dipstick test (Brugia Rapid) in the detection of Brugia malayi infection. Transactions of the Royal Society of Tropical Medicine and Hygiene, 95(6), 601-604. https://doi.org/10.1016/S0035-9203(01)90091-4

@article{2ecc19b252474d2c8d8d723324184955,

title = "Specificity and sensitivity of a rapid dipstick test (Brugia Rapid) in the detection of Brugia malayi infection",

abstract = "A total of 753 serum samples from 6 institutions in 3 countries (Malaysia, Indonesia and India) were used to evaluate an immunochromatographic rapid dipstick test, Brugia Rapid, for diagnosis of Brugia malayi infection. The samples comprised sera from 207 microfilaria-positive individuals and 546 individuals from filaria non-endemic areas. The latter consisted of 70 individuals with soil-transmitted helminth infections, 68 with other helminth infections, 238 with protozoan infections, 12 with bacterial and viral infections and 158 healthy individuals. The dipstick is prepared with a goat anti-mouse antibody control line and a B. malayi recombinant-antigen test line. First, the dipstick is dipped into a well containing diluted patient serum, thus allowing specific anti-filarial antibody in the serum to react with the recombinant antigen. Then the dipstick is placed into an adjacent well containing reconstituted anti-human IgG4-gold. After 10 min, development of 2 red-purplish lines denotes a positive result and one line indicates a negative reaction. The overall results of the evaluation showed 97% sensitivity, 99% specificity, 97% positive predictive value and 99% negative predictive value. Brugia Rapid is thus a promising diagnostic tool for detection of B. malayi infection, and would be especially useful for the brugian filariasis elimination programme.",

keywords = "Brugia malayi, Diagnosis, Dipstick, Evaluation, Filariasis, IgG4, India, Indonesia, Malaysia, Rapid test, Recombinant antigen",

author = "N. Rahmah and S. Taniawati and Shenoy, {R. K.} and Lim, {B. H.} and V. Kumaraswami and Anuar, {A. Khairul} and Hakim, {S. Lokman} and Hayati, {M. I.Noor} and Chan, {B. T.} and M. Suharni and Ramachandran, {C. P.}",

note = "Funding Information: Acknowledgements This study was funded by the Malaysian Government{\textquoteright}s National BiotechnologyD irectorate developmentg rant, No. 0205MBDBCC02 and the Centre for Medical Innovations and Technology Developmento f Universiti Sains Malaysia. We also acknowledget he valuable technical contributionso f the following people: Rohana Ab. Rahman, Ahmad Dasuki, Khatijah Othman, Dr Yenny Djuardi, Drs Hen Wibowo and Tuan Afifah Tuan Ibrahim.",

year = "2001",

doi = "10.1016/S0035-9203(01)90091-4",

language = "English",

volume = "95",

pages = "601--604",

journal = "Transactions of the Royal Society of Tropical Medicine and Hygiene",

issn = "0035-9203",

publisher = "Oxford University Press",

number = "6",

}

Rahmah, N, Taniawati, S, Shenoy, RK, Lim, BH, Kumaraswami, V, Anuar, AK, Hakim, SL, Hayati, MIN, Chan, BT, Suharni, M & Ramachandran, CP 2001, 'Specificity and sensitivity of a rapid dipstick test (Brugia Rapid) in the detection of Brugia malayi infection', Transactions of the Royal Society of Tropical Medicine and Hygiene, vol. 95, no. 6, pp. 601-604. https://doi.org/10.1016/S0035-9203(01)90091-4

TY - JOUR

T1 - Specificity and sensitivity of a rapid dipstick test (Brugia Rapid) in the detection of Brugia malayi infection

AU - Rahmah, N.

AU - Taniawati, S.

AU - Shenoy, R. K.

AU - Lim, B. H.

AU - Kumaraswami, V.

AU - Anuar, A. Khairul

AU - Hakim, S. Lokman

AU - Hayati, M. I.Noor

AU - Chan, B. T.

AU - Suharni, M.

AU - Ramachandran, C. P.

N1 - Funding Information: Acknowledgements This study was funded by the Malaysian Government’s National BiotechnologyD irectorate developmentg rant, No. 0205MBDBCC02 and the Centre for Medical Innovations and Technology Developmento f Universiti Sains Malaysia. We also acknowledget he valuable technical contributionso f the following people: Rohana Ab. Rahman, Ahmad Dasuki, Khatijah Othman, Dr Yenny Djuardi, Drs Hen Wibowo and Tuan Afifah Tuan Ibrahim.

PY - 2001

Y1 - 2001

N2 - A total of 753 serum samples from 6 institutions in 3 countries (Malaysia, Indonesia and India) were used to evaluate an immunochromatographic rapid dipstick test, Brugia Rapid, for diagnosis of Brugia malayi infection. The samples comprised sera from 207 microfilaria-positive individuals and 546 individuals from filaria non-endemic areas. The latter consisted of 70 individuals with soil-transmitted helminth infections, 68 with other helminth infections, 238 with protozoan infections, 12 with bacterial and viral infections and 158 healthy individuals. The dipstick is prepared with a goat anti-mouse antibody control line and a B. malayi recombinant-antigen test line. First, the dipstick is dipped into a well containing diluted patient serum, thus allowing specific anti-filarial antibody in the serum to react with the recombinant antigen. Then the dipstick is placed into an adjacent well containing reconstituted anti-human IgG4-gold. After 10 min, development of 2 red-purplish lines denotes a positive result and one line indicates a negative reaction. The overall results of the evaluation showed 97% sensitivity, 99% specificity, 97% positive predictive value and 99% negative predictive value. Brugia Rapid is thus a promising diagnostic tool for detection of B. malayi infection, and would be especially useful for the brugian filariasis elimination programme.

AB - A total of 753 serum samples from 6 institutions in 3 countries (Malaysia, Indonesia and India) were used to evaluate an immunochromatographic rapid dipstick test, Brugia Rapid, for diagnosis of Brugia malayi infection. The samples comprised sera from 207 microfilaria-positive individuals and 546 individuals from filaria non-endemic areas. The latter consisted of 70 individuals with soil-transmitted helminth infections, 68 with other helminth infections, 238 with protozoan infections, 12 with bacterial and viral infections and 158 healthy individuals. The dipstick is prepared with a goat anti-mouse antibody control line and a B. malayi recombinant-antigen test line. First, the dipstick is dipped into a well containing diluted patient serum, thus allowing specific anti-filarial antibody in the serum to react with the recombinant antigen. Then the dipstick is placed into an adjacent well containing reconstituted anti-human IgG4-gold. After 10 min, development of 2 red-purplish lines denotes a positive result and one line indicates a negative reaction. The overall results of the evaluation showed 97% sensitivity, 99% specificity, 97% positive predictive value and 99% negative predictive value. Brugia Rapid is thus a promising diagnostic tool for detection of B. malayi infection, and would be especially useful for the brugian filariasis elimination programme.

KW - Brugia malayi

KW - Diagnosis

KW - Dipstick

KW - Evaluation

KW - Filariasis

KW - IgG4

KW - India

KW - Indonesia

KW - Malaysia

KW - Rapid test

KW - Recombinant antigen

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U2 - 10.1016/S0035-9203(01)90091-4

DO - 10.1016/S0035-9203(01)90091-4

M3 - Article

C2 - 11816429

AN - SCOPUS:18244406041

SN - 0035-9203

VL - 95

SP - 601

EP - 604

JO - Transactions of the Royal Society of Tropical Medicine and Hygiene

JF - Transactions of the Royal Society of Tropical Medicine and Hygiene

IS - 6

ER -

Specificity and sensitivity of a rapid dipstick test (Brugia Rapid) in the detection of Brugia malayi infection

Abstract

Keywords

UN SDGs

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