TY - JOUR
T1 - Soybean extract increases telomerase reverse transcriptase protein expression in pancreatic β-cells of diabetes mellitus-induced rats
AU - Mustofa, Muhammad Samsul
AU - Suyatna, Franciscus Dhyanagiri
AU - Sadikin, Mohamad
AU - Pujianto, Dwi Ari
AU - Royhan, Aan
AU - Suwardji, Kenconoviati
N1 - Funding Information:
Med J. 嘃吃唃吁唃専ଃ嘂ఁ崃堂ȃ唃吃圁? Funding Sources 唃圁E? tuk EU. Animals models for studying diabetes mellitus. Agric This study was funded by Grant from Directorate General of Biol J N Am. 嘃吃唃吁唂ଃ嘂ఁ唃圃吂ȃ堁? ?igher Education, Ministry of National Education No. 吃吃嬃娂?WR-嘂?唃堁P? rochazkova D, Bousova ?, Wilhelmova N. B?A/V/ 嘃吃唃圁? and prooxidant properties of flavonoids. 嘃吃唃唁尃嘂ଃ堂ఁ夃唃圂ȃ嘃圁?
Funding Information:
The authors would like to thank Ministry of Research, Technology and Higher Education of the Republic of Indonesia which has funded this study. Funding Sources This study was funded by Grant from Directorate General of Higher Education, Ministry of National Education No. 0076/WR-2/ BIA/V/2013.
Publisher Copyright:
© 2019 Authors.
PY - 2019/9
Y1 - 2019/9
N2 - BACKGROUND A number of studies on the benefits of soybean (Glycine max (L.) Merr) in the treatment of diabetes mellitus (DM) have already been conducted; however, the effects of soybean extracts on telomerase reverse transcriptase (TERT) expression in improving telomerase activity in pancreatic cells is currently unknown. This study was aimed to evaluate the effects of soybean extracts on TERT protein expression in the pancreatic β-cells of rats with DM. METHODS Sixty male Sprague-Dawley rats were randomly divided into six groups(1) negative control (N); (2) DM rats induced by alloxan (DMA); (3) DM rats with glibenclamide (DMG; positive control); (4) DM rats with 1 mg/kgBW/day soybean extract (DM1E); (5) DM rats with 5 mg/kgBW/day soybean extract (DM5E); (6) DM rats with 25 mg/kgBW/day soybean extract (DM25E). The treatments were carried out over 28 days. The measured variables included fasting blood glucose (FBG) level, TERT protein expression, and the number of pancreatic β-cells. RESULTS All parameters were measured against the diabetes control group. The FBG levels in rats DM1E, DM5E, and DM25E were significantly reduced on the 28th day (p < 0.05). TERT protein expression and the number of pancreatic β-cells (DM25E) also showed significant improvements compared to DM rats (p < 0.05). CONCLUSIONS Soybean extracts can increase TERT protein expression in pancreatic β-cells in diabetes-induced rats.
AB - BACKGROUND A number of studies on the benefits of soybean (Glycine max (L.) Merr) in the treatment of diabetes mellitus (DM) have already been conducted; however, the effects of soybean extracts on telomerase reverse transcriptase (TERT) expression in improving telomerase activity in pancreatic cells is currently unknown. This study was aimed to evaluate the effects of soybean extracts on TERT protein expression in the pancreatic β-cells of rats with DM. METHODS Sixty male Sprague-Dawley rats were randomly divided into six groups(1) negative control (N); (2) DM rats induced by alloxan (DMA); (3) DM rats with glibenclamide (DMG; positive control); (4) DM rats with 1 mg/kgBW/day soybean extract (DM1E); (5) DM rats with 5 mg/kgBW/day soybean extract (DM5E); (6) DM rats with 25 mg/kgBW/day soybean extract (DM25E). The treatments were carried out over 28 days. The measured variables included fasting blood glucose (FBG) level, TERT protein expression, and the number of pancreatic β-cells. RESULTS All parameters were measured against the diabetes control group. The FBG levels in rats DM1E, DM5E, and DM25E were significantly reduced on the 28th day (p < 0.05). TERT protein expression and the number of pancreatic β-cells (DM25E) also showed significant improvements compared to DM rats (p < 0.05). CONCLUSIONS Soybean extracts can increase TERT protein expression in pancreatic β-cells in diabetes-induced rats.
KW - Diabetes mellitus
KW - Rats
KW - Soybeans
KW - Telomerase reverse transcriptase
UR - http://www.scopus.com/inward/record.url?scp=85073742636&partnerID=8YFLogxK
U2 - 10.13181/mji.v28i3.1732
DO - 10.13181/mji.v28i3.1732
M3 - Article
AN - SCOPUS:85073742636
SN - 0853-1773
VL - 28
SP - 207
EP - 214
JO - Medical Journal of Indonesia
JF - Medical Journal of Indonesia
IS - 3
ER -
