TY - JOUR
T1 - Simultaneously extraction of caffeine and chlorogenic acid from Coffea canephora bean using natural deep eutectic solvent-based ultrasonic assisted extraction
AU - Syakfanaya, Adisya Miftah
AU - Saputri, Fadlina Chany
AU - Mun'im, Abdul
N1 - Publisher Copyright:
© 2019 Phcog.Net.
PY - 2019/4/1
Y1 - 2019/4/1
N2 -
Background: NADES is an alternative solvent in the extraction of metabolites from plants which has many environmental benefits, such as low toxicity, biodegradability, can dissolve polar and non-polar compounds, low costs and simple preparation. Objective: This study aims to determine the effect of natural deep eutectic solvent-based ultrasonic-assisted extraction (NADES-UAE) on enrichment of caffeine and chlorogenic acid in extract from green coffee beans (Coffea canephora). Methods: The powders were extracted using NADES-UAE method in several types of extraction condition, including the composition of NADES, water addition in NADES and extraction time. Caffeine and chlorogenic acid content were analyzed using HPLC, reverse phase system and C
18
ODS-3 column. Results: The highest of caffeine and chlorogenic acid content was respectively, 7.89 mg/g and 28.62 mg/g (composition of NADES betaine: sorbitol [1:1.2] ratio and NADES-water addition [1:2] ratio for 30 min). Conclusion: This research showed that the composition of NADES, extraction time and water addition are important parameter in extracting caffeine and chlorogenic acid content in green coffee beans.
AB -
Background: NADES is an alternative solvent in the extraction of metabolites from plants which has many environmental benefits, such as low toxicity, biodegradability, can dissolve polar and non-polar compounds, low costs and simple preparation. Objective: This study aims to determine the effect of natural deep eutectic solvent-based ultrasonic-assisted extraction (NADES-UAE) on enrichment of caffeine and chlorogenic acid in extract from green coffee beans (Coffea canephora). Methods: The powders were extracted using NADES-UAE method in several types of extraction condition, including the composition of NADES, water addition in NADES and extraction time. Caffeine and chlorogenic acid content were analyzed using HPLC, reverse phase system and C
18
ODS-3 column. Results: The highest of caffeine and chlorogenic acid content was respectively, 7.89 mg/g and 28.62 mg/g (composition of NADES betaine: sorbitol [1:1.2] ratio and NADES-water addition [1:2] ratio for 30 min). Conclusion: This research showed that the composition of NADES, extraction time and water addition are important parameter in extracting caffeine and chlorogenic acid content in green coffee beans.
KW - Caffeine
KW - Chlorogenic acid
KW - Coffea canephora
KW - Natural deep eutectic solvent
KW - Ultrasonic assisted extraction
UR - http://www.scopus.com/inward/record.url?scp=85064083489&partnerID=8YFLogxK
U2 - 10.5530/pj.2019.11.41
DO - 10.5530/pj.2019.11.41
M3 - Article
AN - SCOPUS:85064083489
SN - 0975-3575
VL - 11
SP - 267
EP - 271
JO - Pharmacognosy Journal
JF - Pharmacognosy Journal
IS - 2
ER -