Simultaneous quantification of losartan and active metabolite in human plasma by liquid chromatography-tandem mass spectrometry using irbesartan as internal standard

Budi Prasaja, Lucy Sasongko, Yahdiana Harahap, Hardiyanti, Windy Lusthom, Matthew Grigg

Research output: Contribution to journalArticlepeer-review

32 Citations (Scopus)

Abstract

A simple and sensitive liquid chromatography-tandem mass spectrometry (LC-MS/MS) method employing electronspray ionization was developed and validated for quantification of losartan and its carboxylic acid metabolite in human plasma using irbesartan as internal standard (IS). Following a simple pretreatment procedure, the analytes were separated using a gradient mobile phase on reverse phase C18 column. Selected reaction monitoring was specific for losartan, losartan acid and irbesartan. The method validation demonstrated the specificity, lower limit of quantification, accuracy and precision of measurements. The assay exhibited a linear dynamic range of 2.0-400 ng/mL for losartan and 1.85-370 ng/mL for losartan acid. A run time of 3.5 min for each sample made it possible to analyze more than 200 samples per day. The validated method has been successfully used to analyze human plasma samples for application in bioavailability/bioequivalence studies.

Original languageEnglish
Pages (from-to)862-867
Number of pages6
JournalJournal of Pharmaceutical and Biomedical Analysis
Volume49
Issue number3
DOIs
Publication statusPublished - 5 Apr 2009

Keywords

  • Deproteination
  • LC-MS/MS
  • Losartan
  • Losartan acid
  • Simple extraction

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