TY - JOUR
T1 - Simultaneous Natural Deep Eutectic Solvent-Based Ultrasonic-Assisted Extraction of Bioactive Compounds of Cinnamon Bark and Sappan Wood as a Dipeptidyl Peptidase IV Inhibitor
AU - Ahmad, Islamudin
AU - Arifianti, Ayun Erwina
AU - Sakti, Aditya Sindu
AU - Saputri, Fadlina Chany
AU - Mun'im, Abdul
N1 - Funding Information:
Acknowledgments: This research was supported by Universitas Indonesia (Master’s Thesis Grant 2020). The authors would like to thank the Faculty of Pharmacy, Universitas Indonesia, for laboratory equipment.
Funding Information:
Funding: This research was financially funded by the Ministry of Research and Technology/National Research and Innovation Agency through Penelitian Tesis Magister Contract No: NKB-458/UN2.RST/HKP.05.00/2020.
Publisher Copyright:
© 2020 by the authors.
PY - 2020/8/23
Y1 - 2020/8/23
N2 - Cinnamon bark (Cinnamomum burmannii) and sappan wood (Caesalpinia sappan) have been reported to be beneficial for Type-2 Diabetes Mellitus (T2DM) and the combination is commonly used by Indonesian herbal industries. In the present study, the simultaneous extraction of bioactive compounds from both plants was conducted using natural deep eutectic solvent (NADES), their content analyzed using high-performance liquid chromatography (HPLC), and their dipeptidyl peptidase IV (DPP IV) inhibitory activity evaluated. An additional in silico molecular docking analysis was conducted to ensure their activity. The results showed that NADES (with a composition of choline chloride-glycerol) extraction from cinnamon and sappan wood had DPP IV inhibitory activity of 205.0 and 1254.0 µg/mL, respectively. Brazilin as a marker substance from sappan wood was responsible for the DPP IV inhibitory activity, while none of the marker substances chosen for cinnamon bark (trans-cinnamaldehyde, coumarin, and trans-cinnamic acid) were found to have significant DPP IV inhibitory activity. These results were confirmed by molecular docking conducted in brazilin, trans-cinnamaldehyde, coumarin, and trans-cinnamic acid.
AB - Cinnamon bark (Cinnamomum burmannii) and sappan wood (Caesalpinia sappan) have been reported to be beneficial for Type-2 Diabetes Mellitus (T2DM) and the combination is commonly used by Indonesian herbal industries. In the present study, the simultaneous extraction of bioactive compounds from both plants was conducted using natural deep eutectic solvent (NADES), their content analyzed using high-performance liquid chromatography (HPLC), and their dipeptidyl peptidase IV (DPP IV) inhibitory activity evaluated. An additional in silico molecular docking analysis was conducted to ensure their activity. The results showed that NADES (with a composition of choline chloride-glycerol) extraction from cinnamon and sappan wood had DPP IV inhibitory activity of 205.0 and 1254.0 µg/mL, respectively. Brazilin as a marker substance from sappan wood was responsible for the DPP IV inhibitory activity, while none of the marker substances chosen for cinnamon bark (trans-cinnamaldehyde, coumarin, and trans-cinnamic acid) were found to have significant DPP IV inhibitory activity. These results were confirmed by molecular docking conducted in brazilin, trans-cinnamaldehyde, coumarin, and trans-cinnamic acid.
KW - Cinnamon bark
KW - dipeptidyl peptidase IV
KW - natural deep eutectic solvent
KW - sappan wood
KW - ultrasonic-assisted extraction
UR - http://www.scopus.com/inward/record.url?scp=85089930724&partnerID=8YFLogxK
U2 - 10.3390/molecules25173832
DO - 10.3390/molecules25173832
M3 - Article
C2 - 32842548
AN - SCOPUS:85089930724
SN - 1420-3049
VL - 25
JO - Molecules (Basel, Switzerland)
JF - Molecules (Basel, Switzerland)
IS - 17
M1 - 3832
ER -
