TY - JOUR
T1 - Simultaneous identification and quantification of hydroquinone, tretinoin and betamethasone in cosmetic products by isocratic reversed phase high performance liquid chromatography
AU - Maggadani, Baitha Palanggatan
AU - Harmita,
AU - Harahap, Yahdiana
AU - Hutabalian, Hana Lili Natasya
N1 - Funding Information:
The author wish to acknowledge the grants PITTA 2018 provided by the Directorate of Research and Community Engagement, Universitas Indonesia for this study. The funder had no role in study design, data collection and analysis, decision to publish or preparation of the manuscript.
Publisher Copyright:
© 2019 The Authors.
PY - 2019/5/1
Y1 - 2019/5/1
N2 - Objective: The objective of this study was to obtain a simple and selective analysis method for determination of hydroquinone, tretinoin and betamethasone in whitening creams using reversed-phase high-performance liquid chromatography (HPLC). Methods: Reverse Phase HPLC was used for method development, validation studies, and sample analysis. The method was optimized by evaluating several parameters that affects the extraction of the sample, composition and types of mobile phase and also flow rate. Chromatographic separation was optimized on a C18 column [Sunfire, 250 x 4.6 mm, 5 ^iml utilizing a mobile phase consisting acetonitrile, methanol (90:10 v/v) and slightly addition of glacial acetic acid to reach pH 5in the ratio of 30: 50:20 v/v at a flow rate of 0.8 ml/min with UV detection at 270 nm and 350 nm. Results: The analytical methods fulfilled the validation requirements including accuracy, precision, linearity, selectivity, detection limits, and quantitation limits. The results showed the mean levels of hydroquinone, tretinoin and betamethasone in samples A and B were 1.78%: 0.07%: 0.12% and 2.00%: 0.07%: 0.13% respectively. Conclusion: The method was successfully applied for the determination of cosmetic formulation containing hydroquinone, tretinoin and betamethasone simultaneously. There were seven samples analyzed and two samples were positive containing hydroquinone, tretinoin, and betamethasone.
AB - Objective: The objective of this study was to obtain a simple and selective analysis method for determination of hydroquinone, tretinoin and betamethasone in whitening creams using reversed-phase high-performance liquid chromatography (HPLC). Methods: Reverse Phase HPLC was used for method development, validation studies, and sample analysis. The method was optimized by evaluating several parameters that affects the extraction of the sample, composition and types of mobile phase and also flow rate. Chromatographic separation was optimized on a C18 column [Sunfire, 250 x 4.6 mm, 5 ^iml utilizing a mobile phase consisting acetonitrile, methanol (90:10 v/v) and slightly addition of glacial acetic acid to reach pH 5in the ratio of 30: 50:20 v/v at a flow rate of 0.8 ml/min with UV detection at 270 nm and 350 nm. Results: The analytical methods fulfilled the validation requirements including accuracy, precision, linearity, selectivity, detection limits, and quantitation limits. The results showed the mean levels of hydroquinone, tretinoin and betamethasone in samples A and B were 1.78%: 0.07%: 0.12% and 2.00%: 0.07%: 0.13% respectively. Conclusion: The method was successfully applied for the determination of cosmetic formulation containing hydroquinone, tretinoin and betamethasone simultaneously. There were seven samples analyzed and two samples were positive containing hydroquinone, tretinoin, and betamethasone.
KW - Betamethasone
KW - HPLC reversed phase
KW - Hydroquinone
KW - Optimization
KW - Tretinoin
KW - Validation
KW - Whitening cream
UR - http://www.scopus.com/inward/record.url?scp=85066939411&partnerID=8YFLogxK
U2 - 10.22159/ijap.2019v11i3.32297
DO - 10.22159/ijap.2019v11i3.32297
M3 - Article
AN - SCOPUS:85066939411
SN - 0975-7058
VL - 11
SP - 181
EP - 185
JO - International Journal of Applied Pharmaceutics
JF - International Journal of Applied Pharmaceutics
IS - 3
ER -