Abstract
Aim: Since culture method is time-consuming and has low sensitivity, we developed a duplex PCR (dPCR) assay for the detection of Legionella sp. and L. pneumophila in cooling tower samples. We used culture method as a gold standard. Methods: Optimization of dPCR method was performed to obtain an assay with high sensitivity and specificity. The optimized method was used to detect Legionella sp. dan L. pneumophila in 9 samples obtained from 9 buildings in Jakarta. For culture method, the bacteria were grown or isolated on selective growth factor supplemented-buffered charcoal yeast extract (BCYE) media. Results: Of 9 samples tested by dPCR assay, 6 were positive for Legionella species,1 was positive for L. pneumophila, and 2 showed negative results. For the same samples, no Legionella sp. was detected by the culture method. Conclusion: dPCR assay was much more sensitive than the culture method and was potentially used as a rapid, specific and sensitive test for routine detection of Legionella sp. dan for L. pneumophila in water samples.
Original language | English |
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Pages (from-to) | 223-227 |
Number of pages | 5 |
Journal | Medical Journal of Indonesia |
Volume | 19 |
Issue number | 4 |
DOIs | |
Publication status | Published - Nov 2010 |
Keywords
- 16S-rRNA gene
- BCYE media
- Mip gene