Simple and Sensitive Paper-based Colorimetric Biosensor for Determining Total Polyphenol Content of the Green Tea Beverages

Mochammad Amrun Hidayat, Diah Ayu Maharani, Djoko Agus Purwanto, Bambang Kuswandi, Mochammad Yuwono

Research output: Contribution to journalArticlepeer-review

12 Citations (Scopus)

Abstract

A simple paper-based colorimetric biosensor based on immobilized tyrosinase and 3-methyl-2-benzothiazolinone hydrazone (MBTH) was developed for assessing total polyphenol content (TPC) of various green tea beverages. The patterned biosensing zone of the paperbased biosensor showed a sensitive response to catechin (a typical green tea polyphenol) by generating pink color adducts that can be captured for further color image analysis using a scanometric method. This color change can be connected to the TPC of the samples. The analytical performance of the biosensor in scanometric set-up was optimized. The biosensor showed a response time of 13 min in the linear range between 0.08-1.03 mM of catechin (r = 0.9979). The detection limit (LOD) of the biosensor was 0.071 mM while reproducibility was found at 3.11% RSD (relative standard deviation). TPC of green tea beverages was assessed by the biosensor, and the results were in accordance with the UV/Vis spectrophotometric method.

Original languageEnglish
Pages (from-to)255-263
Number of pages9
JournalBiotechnology and Bioprocess Engineering
Volume25
Issue number2
DOIs
Publication statusPublished - 1 Mar 2020

Keywords

  • biosensor
  • green tea
  • MBTH
  • scanometric
  • TPC
  • tyrosinase

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