An unhealthy lifestyle could trigger increased levels of cholesterol, hypertension, and obesity, even atherosclerosis. Atherosclerosis is hardening and tightening of the arteries that cause the blocking of blood circulation. Anti-atherosclerosis herbs consist of tanjung leaf (Mimusops elengi L.), starfruit leaf (Averrhoa carambola L.), and temulawak (Curcuma xanthorrhiza R.) are empirically believed has antioxidant, anti-cholesterol, antiplatelets, and anti- hyperglycemic activity. Since anti-atherosclerosis herbs are going to be mass-produced, packaging and herbs stability must be considered carefully to preserve flavonoid content within. Therefore, the expired date information on food packaging is mandatory according to UU No.18/2012 about food and PP No. 69/1999 about food labeling and advertising. The shelf life of anti-atherosclerosis herbs was estimated by accelerated shelf-life test (ASLT) method based on the critical water content approach and sorption isotherms model (Labuza equation). In the study, the organoleptic test and mold test were carried out as well to determine critical condition. Packaging permeability was a parameter in the Labuza equation and was measured by the gravimetric method. The results of this study, the shelf life of anti-atherosclerosis herbs was predicted to be 233 days in filter bag and aluminum foil packaging when it stored at a temperature of 30°C and relative humidity (RH) of 75%.