Serological Evidence of Japanese Encephalitis Virus Circulation in Asian Children From Dengue-Endemic Countries

Joshua Nealon, Anne Frieda Taurel, Sutee Yoksan, Annick Moureau, Matt Bonaparte, Luong Chan Quang, Maria R. Capeding, Sri Rezeki Hadinegoro, Danaya Chansinghakul, Alain Bouckenooghe, ARI PRAYITNO

Research output: Contribution to journalArticlepeer-review

21 Citations (Scopus)


Background. Japanese encephalitis virus (JEV) is a zoonotic, mosquito-borne flavivirus, distributed across Asia. Infections are mostly mild or asymptomatic, but symptoms include neurological disorders, sequelae, and fatalities. Data to inform control strategies are limited due to incomplete case reporting. Methods. We used JEV serological data from a multicountry Asian dengue vaccine study in children aged 2-14 years to describe JEV endemicity, measuring antibodies by plaque reduction neutralization test (PRNT50). Results. A total 1479 unvaccinated subjects were included. A minimal estimate of pediatric JEV seroprevalence in dengue-naive individuals was 8.1% in Indonesia, 5.8% in Malaysia, 10.8% in the Philippines, and 30.7% in Vietnam, translating to annual infection risks varying from 0.8% (in Malaysia) to 5.2% (in Vietnam). JEV seroprevalence and annual infection estimates were much higher in children with history of dengue infection, indicating cross-neutralization within the JEV PRNT50 assay. Conclusions. These data confirm JEV transmission across predominantly urban areas and support a greater emphasis on JEV case finding, diagnosis, and prevention.

Original languageEnglish
Pages (from-to)375-381
Number of pages7
JournalJournal of Infectious Diseases
Issue number3
Publication statusPublished - 9 Jan 2019


  • Japanese
  • encephalitis
  • epidemiology
  • flavivirus
  • seroepidemiologic studies


Dive into the research topics of 'Serological Evidence of Japanese Encephalitis Virus Circulation in Asian Children From Dengue-Endemic Countries'. Together they form a unique fingerprint.

Cite this